FB2025_01 , released February 20, 2025
Clone: Dmel\IP05359
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General Information
Symbol
Dmel\IP05359
Species
D. melanogaster
Name
IP05359
FlyBase ID
FBcl0346707
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG13428, Dmel\CG13427
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    IP_cDNA
    • IP cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      740
      NCBI
      EL873512
      3' sequence dataSequence Downloader
      AATCTTTGACTTAAAGGCCAGGCGTCTTTATTTTATTAAATTCGGTTACAAATAGCTGCTTAAAATACGGTCTACCAGTA
GCGTCGGCCCTGGTTGTAGTACGGATAGGGCTGTGCGTATCCGGGGTACTGTCTTGGAGCCACCTGCTCCACAATCACTG
GCTGCACCACCACGGTCTTGGGCTGGAACAATTTGCTCAGGAGATGGCGTGGACCGCGTATGCTGTTAGAGACTTGGTCT
GCCGACGTCGGGGGATCTGCTGCTGCGGCCAGTGGAGCAACATGCTGCATATCAGCTGGCACAAGAACATTTTTATCGTC
GTTATCAGGAAATGCCAAAACCATCGCAATGGTGAGGCTGCTCAGCAGAAGAATGGCCACGAACTTCATCTCGAAAGGTG
TGTGTGCGTGTTGTATGATCTGTTGGCAAATGTAAACTGATTTCCGAAGAGCACAGACTCACCTTAAATAGGAGCAATAG
ATGGTGCAGGTAGAACAGGTTTTCTTCTCTACCTGTCACAGGATTATGGCCCATTATCCCGAGAGCAGCTGCACAAGGTG
ATTAGCCCGTTGAATTTCCCAGAAAATTACCCTCTATGGTTTATGGTTAATGGCTTAAGGAAATTCGGGTGTGGTCATTT
TTGGAATGCTTCAAACTACTTTTATTAGCTTCGATGTGGTTCCCAAATACATGCACAAACCCCCAACTTTATGCTTTACA
TTACTTTAGAGAAGGAACTG
      5' Sequence
      Total bases
      797
      NCBI
      EL873513
      5' sequence dataSequence Downloader
      CGGAAATGAAGCTACTCTGCGTTGTTTTGGTTATTTACGTTGTGGCTCTGACCCTCTTCGGAGTCGATGGACGTGGTGGG
GGCACCACAAATGGAGTGGAGGAACCAAAGTTCGTCGGTTTGTTCAGGCGGGCAAGGCGCCATGTCCCCGCAATGAAAAC
CGCCCTTCTTCACGGCAACGAACCGCCTCCACCTCCGAAATTCCGATCCCGTCGTGATACATTGGAGGACAAGCTCGTGG
CCAATGATGATCCTCCACCACCGCCGCAGTTCAGACAACGTCGTCAGGCTCCACCTGGAATGCCACCACCGCCGGACGGC
CTTCCACCACCACCCCAGTTCCTTCTCTAAAGTAATGTAAAGCATAAAGTTGGGCGTTTGTGCATGTATTTGGGAACCAC
ATCGAAGCTAATAAAAGTAGTTTGAAGCATTCCAAAAATGACCACACCCGAATTTCCTTAAGCCATTAACCATAAACCAT
AGAGGGTAATTTTCTGGGAAATTCAACGGGCTAATCACCTTGTGCAGCTGCTCTCGGGATAATGGGCCATAATCCTGTGA
CAGGTAGAGAAGAAAACCTGTTCTACCTGCACCATCTATTGCTCCTATTTAAGGTGAGTCTGTGCTCTTCGGAAATCAGT
TTACATTTGCCAACAGATCATACAACACGCACACACACCTTTCGAGATGAAGTTCGTGGCCATTCTTCTGCTGAGCAGCC
TCACCATTGCGATGGGTTTGGCATTTCCTGATAACGACGATAAAAATGTTCTTGTGCCAGCTGATATGCAGCATGTT
      Library Information (1)
      Library: IP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      IP
      IP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)