TTAAGGACTGACTTTAAAAAGGAAGATGAAATTAATAGATTGATTGAGGTTTTATCAATTAAACATTTGGAAACATATAC AGTTAAAATGCCTTTTGGTAATTACCCCACTTCATCGCTTAACCAATCCCACCTCGACCACAGGAAAGAAAGAAAATATG AACACCTGATGCAACCGCATACTGAAAAAAAAAACGAAGATCATAACCAAAAAAGTAGATCCGACCACAAGCAAGTCTTA GCGTCCGTTACGTATTACGGCTTCGCTGATTTTACCACCATTGTCGGTGATAGCGTAATTGTTTTTTCTCCTAGTACTAC ACAAAGCATTCTGCATTACGGACACGTTACATCAATAAAAGGCAAGCCCACTCTACAACATGATTTAATTCCCGAAGAAA TCGCCCAGACTTCTAAAATATCACTGAGTACTTTAAAGATGGGTACGAGTTCCGGAAAATATGAAGAATTTGTGTCAAAT ATTAGTGCTAGTAAAATGTTTGATAAAAATGAATTGAAAAACTTATCAGTTTTGAAACCAACAAACCCGTCACCTACATT TGCCAACAATTCATTTAACGAAGAGCAGGAAGCCAGTTCGGTGTTATCTAAAGATATAGTTCCATTTGTCTCCAAAGCAT TTAATGATATTATATCACCCTCATTCGTTAATGGAGTGAAAGAAGGAACGAAGCACAATGAACCTAGCATTATAAAACAG GAAGGTGCTACAACTGTATTTATTGATGATGACCCCTTTACTAGTTTTGATGACCTTAGTGGTTCAACTAGCTTAAAATT AAATTCCGCCAACGAAATAAAATCTATTAAATCTTTTGAAATGTCTAAGAGTGATGAAATACGTGAGATCGTGGCATATC AAACAAATATTAACTTTACGAAAGACAGCTTGGACATTAATCCACTTTTTGAAACAAGTAATGAAAATCACGACTTCGAA GAATTATGCAACCATACAACTTCACAAGTTTTTCTTACTCAAATGAGTAAGGCAAACATAAGCAATGAAAAAAACAGTAT GGATACCATTGATATTGATGCTAATCCACTTCATGCTTATGAGATTGTAGAAACTACCAAATATTATTGCATACAAGCTT CACAATCAAAACAAACCCCAGAATTAGATATCATTGTGACCCACGAAACATTTACTAAATCACGTGGAGAATTCGTGTCC ACTTATTCAATAGATGACGATATAGACGAAACAACGGTTCAAGATTTAGGGGATTATGAAGGGACCACAGAAAACGAATA TGAAAGCGAAGAAGAAGAATATGAAAGTGTTAGTGATGAAGTTGACCTAATATACAAAACTCTATATACGACTTATACAT ATTTGACCACGTTCTTTGAAGGGTCTAGAACAACCATATCTAGCCACACTGAGGTTTTGACAAACATCGTTTCCTCAACA CTTTTGCCTAAAGCTAAATTAAAATCCAACACCTTGAATTATGTTGGAGAGAACCAAATTTCTGACAATACAATTCGCCT GATTGATCATAAAACAACTGAATTTTCGCAGAAATATTCTATACCTGACGAGATTGCTAGTTTGTTAATCCAAGAAAGTA AAGTAAATACTGCTGAGAGTTCTTCACAGATGATGACCACATTACGCGACGATTTAATATATTCTAAGACTTTGTTGACA ACATATACTTATTATACATCGATTTTTACAAATAACGAAACTGAAGTACAATCACGAACGGAGATTGTCACAAATTATAT TACTGACAATGTGTCGAACAATGAAACTAATTTTATACGCAATCCCCAAACTGCAAATTTAAATTTTTTAAAATCCCAGC ATTCTTTAGTCGAGGAAAAAATTGACAAGGTAGTAAATATGGCCTATGGAATGAGTGTAAACTCGAGCATGTTAAGGGAT TCGAGTCCAGCTAGTTTTATTAACCAAAATCTTCTTGATGACCAAGTAAGTTCGGAAAGTAACACAGAGGAGATTATACC GTCAGCAACGTTCCTTCTTCAAACAAGTTTTACAACATTTACCTTCTATACAACTATGTATGTAGGAAATGACACAAGCA TCATAAGTCGACATGAGACTATCACTAATGTAGCTACCGAAGCATTGCACCCTACAAAATTAATAAGTGTAGAAGATTAT TCTTTGCCTATTACTTACTTTACAACATTTACTTATTGGACAAAGTTGGCTAAAGATGGTGAGATTACAACATTAAGCAG AGAGGAAACTTTATCCAATGTTATACAACACAGTAACGGTACAACTTTAACAGTAAACGATTTAAAAACTGCCGATAAGG TATCAAGTCAGTCGACCGCGACTGATAGCGATACCGAGAATGATCGGAATTGTAAGTCTGAAAGTTCCTTGTTTTCCACT TTGGAAAATCAAATTTATCAATCTGACATTACCACGTTCTATACCACCTATACATATTATACTACTTCTTATGACGTTAA TACGACAATTATTGATTCACGTCTCGAAACAGTTACCAATATAATAACATCCAGAGAAATGTCTAATCCAATGGAAGAAA CAGATGCAACAATTTTAATGATTAAACCTAGTCAGCCCATTGCTTATTATGAAAATCCAATCGAAACCATTAAGCCGAAT GTGGTTTTGTATGATTATAAGCAAATAATTGATGCAGAAAATATAAGTACTTTGTATTTTACAACCGAAATTGTATCTTC AATGAATAGTGAGGGATATGCTATTAAATTAACCAGTAGTACATCAAGGTTACACGTTGACCAGTCGAAAAAATCTAGAA TGTCTACGATCTATGGCAAAATTCCTGATGACAGCTTAAATTTGTACAAAACGGGCTTGGTAAGGCTTATCGAAGGAAAA CGCATCCAAAACAGTACAACTACCTTATACCAATCTAGAGTTATTGGAACTCTAATAGAGAATCGATACGCGCAGATAAT TGAAAGTACATCAAGTTTTTTTTTTTGAGAAAACAAAGTCTGAACATATTCTGTTTCCGACAAGCGTCGAAATTTCTGGT ATGATTACCAAAAATGTTGATTTAATTGACGTAATAGAATCTACTAAAAGTGAAAATGATACAACAGTAGCTCCTTATAT CGACCAGAAAAACACGAAGCCAGAATCGGATAACACTGACGGGTTGTCTAATCATTCACCACAAAATGCAAAGAGACCAT TTGCACCAGTAATTCGGCCGTTCGCCTCAAGAAACAGGCCTACATTCGCACCAAAACAAAAAACACTAGTCCCCAGTAGC GCTACTATTATTACAAGATCGGACATCACACCGACTATAACGGCAACACCTGCTTTAAAAGCAGTAGGTAGATATACCGC ATCTCGTCGTGGTATTATTTCTAATGTTCCAATTAATCTTAACGAGCCTAACTTATCACAGAGCAGTCAGTCATCCAGGC GCCTGTTTGGAAGACCAAGTAAGTCAATGTATGACTTAATCGAAGCGAATAGCACTCTGCAGTCGAGCAACGCATTTTCA CCTTCGAAAACCCGGTTTACTTCACCTTTACGACCAGGCGTTAGTTCCAGGCGACCAAATATAAATATTTCATATCGATC ATCAAGTTTTCCTGTGTTTCGTGGGTCTGGAGTAATAGCTAACACAAGGTTACGCATAAAACCAACTAGCATAGGGATAC AATCAAGCTATAGACCAACACAGTCCACAACGTTTGCCACGGAGTCAAGCTCGGAAGATAGCGTTGAAGAGGATACCTCA CCCGATGAAGAATCTGATGTGGAAGAAGGAATCAAGAACAATAACAACAGCCCGTTACTGCGCTTTCGACGACCCATAAA TGCGCCGAGTGGATTTAGTCCTCCAATACGACAGACTGGCAATTCGCCAGTAGTTTCGCTCAGACGAAACCCGCTATCAC CCAGAGCAAAAATTTCGACTACGACAAGCAGTACAACGACAACGACTGCCAAGCCAAGGGCGCGAAGTTTTCAGAGGCCA ATAGGTCCTCATCCGAGATCTAGACCACAAAACACACTTTTTCCGCCCAGAGGCCTTTTTCAAACTCAGATTAAAAATGA AAACCTAAAGGTACCTAGGACAAACGATAGTGTTCCTAACAATGATTTTGAATATGAAGGAGGTGTCGAAGATCAAGATC TGGATGAAAATATTCATCGTAGAAAGCGCTCTATCAAATATTCTGTTCGTTTTACAACTGGAAATCGAGTGCGTCGGCAA GCGGATACAGTCAGAAGAAACAGATTTAGATTTCGTCGACAAAACCAAACAGCAACTACCAAAGTTGAGTCAAAGCTTAT GGATTCCGACAATAATACTGAGCCTATCTTAACCCCTCGGGATAAGTCAAGTTCTAGATTTGGGTCAAGATTTCATTCTC CCCAGGTACATCAGCTATACACGCAGGCATTAATCGCAGAATCACCAACAGCAACCAATCATAGATCAATTCGACCAAGC AGACCGACAACAAAGAGACCACAATTTACGTTAAGGGAGAAAGATGCTAATTTAAAAGGCACTGCATCAAAAAACTTTCG ACGCCAACCAGCTAGTGGAAATTCAAGGCGCCCAGTTGGAAATTCTGGAAATTCAAACAGTCGTAGACTAAAAAGCTATG TAAGCTACAATAAAAATAATTTAGACAACGGTCGACCATCCAGTTCTCCCCGTTCCCGAAATTCGAACTCTAATACATTA AATAGAGGAAGAAGTCGTGGTCGAAATCGAGTCGATTACGCCTCTGATGTACAATCCGTAGAAAATGACCTTCAAACTAT CACAGTTACTCATTATATACCATCTGAGGTCACTGTTCCTGTAGTAAGCGGACATATAACTGAGTACAAAAGCATTGTTA CTGCTAAGTCAAGCACCGAGATCGTAGGTCCAAATGACTTTGCAGTAGTATTGGGTTCAAACGGGATAAGTTCCACGTAC TTAAATCGAGAAACATCTATTATAAATATTGCTGGTGCCACCGAACATACAAAATATTTATTGCATGAATCTATCACAAG CTCTGTAACTTTTACACCTACCACCATTCGCGGAAGAAAGACTTCCTTTTCACATATAATTCCATCCACAGCGTATTCCG TCGAGTATATTGTAACTACAATGCAGCCTCAAATTTCTGAAAACGCACCGCTTGCCAACATTTTACTTTCCCAGTTACTT TTAGGAAACTTAAACTTACCTGCTCATCCCTTGATTGGGGCTGTCGGACAGCAAAACGTCCCATCTACTATAATTCCCAC ATCTGTTGAGCCTATAACTGAGTATCGCACGCACACTTCTACATATGTTACTACAATATTCGACGGAAAGTCTACCATTC TTCCAGTGACATTTCAAGGAAAAAAGATTTTGACTACTGTTTATGACACTACCGCTCAGACTATAACAGCAACAGAATAT AGTGTGGATACAATAATTAACACTCTACCGTCAGTTCAAAGTGTACATACTGTTGGACCGGCTGCTCATGTAAATAATTT GTTATTGCAGCAGTTACTTATTCAACAACAGCATGAGTCACTCGTCCAAGCTATATCCAACCCTACACCACCACAAGTAT TGTTAAGTGAAAACCTGCAAGTTTTGGACGACGGCACACGGACATCAATAAAGTCGGATGCCATCGACATTGAATATGAT TCGGATTTGGTGTCGGTCAGTAATGAGTCCCAACCTACCAAAGGCCATCGAAAAAAATCAAGAAAATCGAGTAGCGGCAA CAAGAGAAGTAAACAGGAACCGAGTGTAATAACACTTTATGTATCGGGCCGCAGACCCGGCGAATTCAGTACTGTATTGT CAACTGTGAAAAACGAACATGATCACTCTGTTTCATTGCAGAAAAGGCACGCGGTTTTAGAAATACAGCCAACCAATTCG ATTAAATATGTTTCTAGCATCGAAAGAAGCAAAAATATAATGGAAAAAAATGACATTTTAAGAGATATAAGAAGTTCTTT AACGATTGACATTGGATTTAACGACTTACCCGATCAAACAGCATCATTAGAAAGCATTGTGGGTGATGTGGATCTTTGGT TTGCAAATTCTAATAGACAATCTTCAACGGTGACAACATTTATAAAATCTGTAGTTGTTTAATTTTTAAATAACCTTTGT TACTTTTAATTAGTTAAATGCTTTTGAACCTTTATTTTACAAAAAAAACTAATCAAACAAGAATATAAGCTAAATAAGTC CTGCCAATCTTTGAAAGATATCCAAATACTAGTCAATGATAAATATAGAATCATTAGGAATTTCTTATCCAACTATGCGC AACAACCTAAGTAACTCTTTCATAATAGGGAAGTCTACCAGCTCAAATCTACGTTTACGGGATTTTTAAAATATTTCAAA TCGTTCTTAATCATGTTAATCGCATTTTTGCTTAAACAGACGATATTTTTGAATAATTGAGATTGAGTATAACATTCTAA TAATAGGATTACAATATTATTACTGAAAAATGTTAAAATTTTTAGCTCCCTAGACGCTTTTCAAGGACAATTATTTTTTT AAATATTTTTCCAGAATTTGTAGTTTAAAAAAAATTTGTCTTAAAAAAAACATATAAAACTTTGTAGCTTATTGTATATA CTTACGTTTATTTCTTCAGTTATGGTTAAATAATAAATATACTGAACCAAACTAAAAAAAAAAAAAAAAAAAAAAA
Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
These clones have been isolated using a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.
