FB2025_01 , released February 20, 2025
Clone: Dmel\MIP29461
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General Information
Symbol
Dmel\MIP29461
Species
D. melanogaster
Name
MIP29461
FlyBase ID
FBcl0742374
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG42489
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
  • Caution: This cDNA clone replaced by FI16919
Library
MIP_cDNA
  • cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
Strain
    Vector
    Tissue Source
    Stage
    Tissue/Position (including subcellular localization)
    Reference
    embryonic stage
    organism

    Comment: 0-22 hr AEL

    (Rubin et al., 2000)
    larval stage
    organism
    (Rubin et al., 2000)
    pupal stage
    organism

    Comment: early pupae

    (Rubin et al., 2000)
    adult stage
    adult head
    (Rubin et al., 2000)
    Sequence Data of the Insert
    Full Length Sequence
    Total bases
    389
    NCBI
    BT126306
    Full Length sequence dataSequence Downloader
    CCCAGTTGTATTCGCGGATCTCAGCTGTGTCAATTTCAGACTGTCAACATATAAACACGACGAGGATGCAGAAGCCACAT
CATGGCCGTGCTGGATGTTGCTTCTACTGGCATCGCTGATCGGTACTTTTATGCTGGCCTTCAGCTACTGGATGTTTGTG
CCGCGCGAGGAGTCACTATGGTCAATGATATCGAACTACAGTGGATCTGGAATAAAGTACTCCCTGCCGCCGTCAGCAGT
ACCTGAGGAACTTAAGCTTCGGCAGAGACCGCCCTTCGTTTACCAGATATTACATTGAAAGTAGAAGTACAATATAGTGT
TTATATGCTATGTAATCTTACGCTAGTTTAACAATAAATTACATGTACACAGAAAAAAAAAAAAAAAAA
    Library Information (1)
    Library: MIP_cDNA
    Description

    Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

    Sample preparation

    Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

    Protocol

    These clones have been isolated using a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

    (FlyBase Curators, 2016-)
    Comment
    Reported As
    Symbol Synonyms
    MIP
    MIP_cDNA
    References (1)
    FlyBase analysis (1)
    FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
    Curation of large dataset metadata by FlyBase. [FBrf0232540]
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (0)
    Reported As
    Symbol Synonym
    Secondary FlyBase IDs
      References (0)