Interaction in vitro; bait produced as tagged protein in transfected HEK293 cells; prey produced from endogenous fly gene and obtained as HPLC fractions of whole fly extracts.
Fractions of whole fly extract were tested for their ability to activate intracellular calcium changes in mth transfected HEK293 cells, but not untransfected cells. Purified active peptides were identified by mass spectrometry.
