Source was larvae of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.
Coimmunoprecipitation used an S120 extract in which plasma membrane and internal membrane compartments were removed by centrifugation, leaving soluble proteins and lipoprotein particles.
Source was larvae of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.
Coimmunoprecipitation used an S120 extract in which plasma membrane and internal membrane compartments were removed by centrifugation, leaving soluble proteins and lipoprotein particles.
An S120 extract, in which plasma membrane and internal membrane compartments were removed by centrifugation, was subjected to Optiprep density gradient fractionation.
Source was larvae of wild-type fly line; proteins produced from endogenous genes.
