FB2025_01 , released February 20, 2025
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
Pi3K21B, B4
FlyBase ID
FBig0000098471
Interaction Network
Interactions Browser links
Pi3K21B network
B4 network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0187416-1.PD.WB
Reference
Wittwer et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Pi3K21B 

P60

bait

GST tag

B4 

Susi

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wittwer et al., 2005)

Pi3K21B binds to both B4 and Pi3K92E in a non-competitive manner. B4 and Pi3K92E do not bind each other.

(Wittwer et al., 2005)
FBrf0187416-2.PD.WB
Reference
Wittwer et al., 2005
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
B4 

Susi

bait

GST tag, HA tag

Pi3K21B 

P60

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of HEK293 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Wittwer et al., 2005)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)