FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Unr, Sxl
FlyBase ID
FBig0000098766
Interaction Network
Interactions Browser links
Unr network
Sxl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0190533-1.CH.MS
Reference
Abaza et al., 2006
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

dSXL

bait

GST tag

Sxl 
Unr 

dUNR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sxl 
RRM domains
sufficient binding region
aa 122-301

coordinates relative to Sxl-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Abaza et al., 2006)
FBrf0190533-2.coIP.A
Reference
Abaza et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

dSXL

bait

[35]S label

Unr 

dUNR

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Abaza et al., 2006)
FBrf0190533-3.coIP.A
Reference
Abaza et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Unr 

dUNR

bait

[35]S label

Sxl 

dSXL

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced and labeled by in vitro translation; prey produced and labeled by in vitro translation.

(Abaza et al., 2006)
FBrf0190533-4.PD.WB
Reference
Abaza et al., 2006
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

dSXL

bait

GST tag

Sxl 
Unr 

dUNR

prey

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sxl 
RRM domains
sufficient binding region
aa 122-301

coordinates relative to Sxl-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Abaza et al., 2006)
FBrf0204405-2.EMSA.A
Reference
Abaza and Gebauer, 2008
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

SXL

neutral component

GST tag

Sxl 

SXL

neutral component

GST tag

Sxl 
Sxl 

SXL

neutral component

GST tag

Sxl 
Unr 

dUNR

neutral component

His tag

Unr 
Unr 
Unr 

dUNR

neutral component

His tag

Unr 

dUNR

neutral component

His tag

Unr 
Unr 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Unr 
interaction domain
sufficient binding region
aa 154-277

coordinates relative to Unr-PA

Unr 
interaction domain residue 198
mutation disrupting interaction
aa 198aa 225aa 237

Y198A, coordinates relative to Unr-PA

K225A, coordinates relative to Unr-PA

D237A, coordinates relative to Unr-PA

Sxl 
RRM1 and RRM2 domain
sufficient binding region
aa 122-301

coordinates relative to Sxl-PD

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as a recombinant fusion proteins in bacterial system.

(Abaza and Gebauer, 2008)

Various combinations of Sxl and Unr were mixed with [32]P labeled RNA. Supershift of RNA-Sxl complex upon addition of Unr which does not bind RNA by itself was taken as evidence of a Sxl-Unr.

(Abaza and Gebauer, 2008)
FBrf0221878-1.PD.WB
Reference
Graindorge et al., 2013
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Sxl 

SXL

bait

GST tag

Sxl 
Unr 

UNR

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sxl 
dRBD4 domain
sufficient binding region
aa 122-301
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

GRAB (GST pull-down and RNA affinity binding) was used to identify components of the Sxl-msl-2 mRNP complex. GST-Sxl and biotinylated msl-2 RNA were mixed with embryonic extract. mRNP complexes were purified by GST-glutathione, then by biotin-streptavidin affinity steps.

(Graindorge et al., 2013)

Positive control.

(Graindorge et al., 2013)

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type embryonic extract.

(Graindorge et al., 2013)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)