FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Arl5, Vps53
FlyBase ID
FBig0000102924
Interaction Network
Interactions Browser links
Arl5 network
Vps53 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0228123-3.PD.MS
Reference
Rosa-Ferreira et al., 2015
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arl5 

Arl5

bait

GST tag

Arl5 
Arl5 
Vps53 

Vps53

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arl5 
catalytic domain
mutation increasing interaction
aa 70

Q70L, GTP-locked

Arl5 
catalytic domain
mutation decreasing interaction
aa 30

T30N, GDP-locked

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from wild-type adult head extract.

(Rosa-Ferreira et al., 2015)

Versions of Arl5 mimicking GTP- and GDP-locked forms were incubated with adult head extracts, and proteins isolated in each condition were compared.

(Rosa-Ferreira et al., 2015)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)