In vitro synthesized miRNAs were injected into embryos before cellularization, and effects on the reporter were assessed at 10 hr AEL.

Luciferase reporter assay.

2\'O-methyl oligoribonucleotides antisense to mir-6-3 leads to derepression of the rpr luciferase reporter.

Source was embryos of transgenic fly line; bait synthesized in vitro; prey produced from transgenic reporter construct.