Source was S2 cell line; bait produced from endogenous gene; prey produced from transfected reporter construct.
GFP reporter assay, anti-GFP western blot.
In vitro synthesized miRNAs were injected into embryos before cellularization, and effects on the reporter were assessed at 10 hr AEL.
Luciferase reporter assay.
Source was embryos of transgenic fly line; bait synthesized in vitro; prey produced from transgenic reporter construct.
Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.
Luciferase reporter assay.
Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.
Luciferase reporter assay.
Positive control.
