In vitro synthesized miRNAs were injected into embryos before cellularization, and effects on the reporter were assessed at 10 hr AEL.

GFP reporter assay.

2\'O-methyl oligoribonucleotides antisense to mir-6-2 leads to derepression of the hid GFP reporter.

Source was embryos of transgenic fly line; bait synthesized in vitro; prey produced from transgenic reporter construct.

In vitro synthesized miRNAs were injected into embryos before cellularization, and effects on the reporter were assessed at 10 hr AEL.

Luciferase reporter assay.

2\'O-methyl oligoribonucleotides antisense to mir-6-2 leads to derepression of the hid luciferase reporter.

Source was embryos of transgenic fly line; bait synthesized in vitro; prey produced from transgenic reporter construct.