FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
Interacting Genes
FlyBase ID
FBig0000116805
Interaction Network
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Arl8 network
Rilpl network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0239971-1.Y2H
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arl8 

Arl8

unspecified role
Arl8 

Arl8

unspecified role
Arl8 

Arl8

unspecified role

CG11448

unspecified role

CG11448

unspecified role

CG11448

unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Two-hybrid system: yeast GAL4-BD/GAL4-AD. CG11448 binds to GTP bound form of Arl8 and not the GDP bound from.

FBrf0239971-2.PD.MS
Description
physical association
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Arl8 

Arl8

bait

GST tag

Arl8 

CG11448

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Arl8 
interaction domain
sufficient binding region
aa 18-186
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

CG11448 binds to GTP bound form of Arl8 and not the GDP bound from.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell extract.

External Crossreferences and Linkouts ( 0 )
References (1)