FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Ogdh1, Zasp52
FlyBase ID
FBig0000122070
Interaction Network
Interactions Browser links
Ogdh1 network
Zasp52 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0256925-2.Y2H
Reference
González Morales et al., 2023
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Zasp52 

Zasp52

bait

fused to GAL4 DNA-binding domain

Zasp52 
Zasp52 
Ogdh1 

OGDH

prey

fused to GAL4 activation domain

Ogdh1 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Zasp52 
LIM2a domain
sufficient binding region
unspecified
Zasp52 
LIM2b domain
sufficient binding region
unspecified
Ogdh1 
interaction domain 741-769
necessary binding region
aa 741-769

coordinates relative to Ogdh-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(González Morales et al., 2023)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(González Morales et al., 2023)
FBrf0256925-3.BiFC.FM
Reference
González Morales et al., 2023
Description
physical association
Assay
Collection
Source/Stage
adult somatic muscle of thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Ogdh1 

OGDH

bait

YFP tag, N-terminal fragment

Ogdh1 
Zasp52 

Zasp52

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Ogdh1 
interaction domain 741-769
necessary binding region
aa 741-769

coordinates relative to Ogdh-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult thoracic muscles; proteins produced from transgenic constructs.

(González Morales et al., 2023)
External Crossreferences and Linkouts ( 0 )
References (1)