FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Otud6, RpS7
FlyBase ID
FBig0000123312
Interaction Network
Interactions Browser links
Otud6 network
RpS7 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0260166-5.coIP.WB
Reference
Villa et al., 2024
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Otud6 

Otud6

bait

FLAG tag

RpS7 

RpS7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

A catalytically inactive form of Otud6 was used along with treatment with cycloheximide to preserve association with the ribosome. Only ubiquitinated RpS7 interacts with Otud6.

(Villa et al., 2024)

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Villa et al., 2024)
FBrf0260166-6.coIP.MS
Reference
Villa et al., 2024
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Otud6 

Otud6

bait

FLAG tag

RpS7 

RpS7

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Villa et al., 2024)

A catalytically inactive form of Otud6 was used.

(Villa et al., 2024)
FBrf0260166-7.CH.WB
Reference
Villa et al., 2024
Description
physical association
Assay
Collection
Source/Stage
head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Otud6 

Otud6

neutral component
Otud6 

Otud6

neutral component
Otud6 

Otud6

neutral component
RpS7 

RpS7

neutral component
RpS7 

RpS7

neutral component
RpS7 

RpS7

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; proteins produced from endogenous genes.

(Villa et al., 2024)

A catalytically inactive form of Otud6 was used along with treatment with cycloheximide to preserve association with the ribosome. Otud6 specifically interacts with RpS7 on the 40S ribosomal subunit.

(Villa et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)