Donor plasmid that can be inserted into a docking site in the genome via phiC31:int-mediated recombination (plasmid contains an attB site), producing a transgene designed to result in an indel (small insertion or deletion mutation) in a target gene when combined with a source of Cas9. Constitutively expresses two sgRNAs, both of which target sequence within the coding region of eff, immediately downstream of the translation initiation site.