Subject: FB Help Mail: 076 Gene data (problem or question)
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Hi,
Tungus was mentioned as supplemental material in the Dubnau et al (2003)
paper. The tungus mutation is produced by a P element insertion, which is
listed in the genomic sequence below (look for the 'P'). In the original
release of Flybase, this sequence was listed as part of CG8242. With the new
release of FlyBase, however, it appears as though our P-element insertion now
lies within nearby CG8253.
Below is the DNA flanking sequence surrounding our P insertion (red 'P')
from line A0469 (tungus).
GGTAGCCTACATATCCTGGCCATGAGCTGCGCTGCCAGCGTGTTAATATTCAGGT
GCTGAAGGCTGGGTGAATGATTTCGGCTGCCTAATGAAAAAGTTTTCCATCTCCG
TGCGGTGCTCTTGTTCTTGTTGTTGTTTTCGTTTGTCGTTGTCTGTTGCTTTTGTTC
CTGCCCATGGACATGGGCATGGGTATATATGTATATTCGCATGAATGGCCATATA
ATCACCAGTAACAGGACACGTAGGAGCAGTCCGATATTTTAGGGAAGAGGAAGT
CCGTGGTCATCTTGATGCGCTCCGGGATCTGCGATGCTGCGATGCTGGTCGCCTG
TGTTGCTATTGTGGCTGTTGCTGTGGGTGCGGGTATCCTTGAGCCAGGACCTGTG
GGTTGATTTGCCGTGGAGGATATGGATATGGAGACGGAGATGGAGCTAATTGTC
ACTGGGGACTGGGAGATTGCACGAGACACGAGAGGAGCTGGTTTATATTATCAC
TGCATTATGCGCCGCTGCCTGGACAAAAGCAGGGTGTGTGTATCCGTATCTGTAA
TCTGTATCTATGTCTATGCGGTGGCATCGGAGGATGCTGGACGCTGGCTAATCCT
TATTCCGATCCTGCTCGTCGCACTGGCACCTGCTCAGCGACATTCCTCCATTCCC
AGGGAGGCTGCGATGCGGATGATGGCCCCTCTGCTATGTTTTTATATTTTTGTTA
TTTCTCCTGTCTTTTTCTGGCTTCCAGGATGCTGCTCACGCACACACTGGCACACG
GGCACGCACACACCGCCACATACACATACACATAPCACTGGGGGCTCTCTCTCG
CAAAGGAGCCGATTTCTCGCTTTCGTTTCGTTTGGTTTCGTTTCGATTTTTGCGAA
TTAAATTATGCTAAGGAGCCAAGGAGCTAAGGATGCTGGAGCTTTTATCCCAGCC
TGCTGCCTTGCAGCTCCTGTTCACTCGTTACGGGATCGCACACCTGACCAAAAAC
AAACGCGCTCATCCCCCGCAGATTTTTCTGTTGCACTGCAGCGGGCGAGGATGGG
GCGGCCTGACCAGGTGACTGAGCCTGGGGCAACACGCACTCAGCGACCCGTGGA
GCTTGTATCACGGCGGAACACTACGTTGGTCTGGGCACCGGAATCCTTGGATGTT
GCAGGCTGCGTGCGTTTTTCGTGGGCGCCTCTATCAGCTTTTCGTTTTTTTCCCTT
CCACGCTCTGGTGGTGGCTTTTCCGCTTCTTTTTCTTTCTTTTTTTCTTTTGCTCGG
CACACTCCGCTCCTTGTGTTGTGTACGCGTTCCCGAGTTCGCACAGTGTGACCCA
AGTTGGCGAGCGCCACAATTCCAGAACAAGTTCTGTGTTTGTTGTGAAAAATGAA
CAAGTTCTGGTTTCCACTGAGCTGAAAGTAAAAGCGCGCGCGATTTGAAAAGCAT
AAGAAACAGTTTGAAGATATAATAAATGTAATAAATACAAGAAACAATAAAAGT
ACAAATATAAACTAATTGGCCACTTAAAAAATAAAGTTTTAATGAATTTAAAGAA
ATATATATATAAAATAAATATTATAAATGGCTAAGCTATTTTATTCTTCTTAAAA
AGGAAATGTATAATTTTTTTTAATAAATAAAAGTATTTTCTTAACCAATTTTAATA
CATGAAAATTCCAAACTTATATTTTCCAATAAGCATTTATTCTTATCGGAAATTAA
TCAATCAGAATCCAATTGAGCGCAGCAAGCAAACAGTGATGCCCATCGCATACG
CACAAAAGCTATTAGCTATGTTGCACGCTGTGAGCTGCTCACTGCTGCGACGCTC
CAAGCTAGCTGACAGCAGGTAATTGCTACAGTCAACGGTTTTTCGAAACACATTT
TGGATGCCACTAGCAAATATTTGTTGCTAACACCTCCGTTAATAATCGCTATCTA
ATCACCCGTGATTGCTAATGCCCAATTGATGGCACGCAATTTGGCAAAGCCAATT
AGTATTGTCTCATTTTTACGGCACTCGACAAGACGACGGCAGGCAAGACGAAAGA
CCTTTCGTTCGATAAGATAAATCTGAAATCTGCGCAGTCCGTTGTTGTGCCATTG
TAAATGCCTCTTGAGAACATTTCCTACACACCCAGGCGACGAACGCACCGCGAGT
TAATCGTCGAAACTGATAAGCTGATATAATAACTATACTATATATATGACAGGTT
GACTTAACCCACCCAGATGGGCCCACAAATGAACCATCGCAAGCGTATGGGCTG
TTAAATGGTTACTTAATGTTTCGTGTCCAACAAGCCGATAAGGACAAAGTGCATC
GGCATATTGTCAACCCTATGCCCATTCAGAAGTCCAAACCGATCAGCAGTATCGC
CCATTATCAGCGATCAAAGCGATCATGACACACATAAAGGCAAATCTATTGGCC
GGATGACGTTCCTAGATGGC
and below is our original, specific plasmid rescue sequence from
 A0469:TTTNANTCCATTAGCANTTCNTGNATCATGCACTGGGGGCTCTNTNTNGNAAAGGAGCCGATTNCTCGCTTTC 
GTTTCGTTTGGTTTNGTTTCGATTTTTGCGAATTAAATTATGCTAAGGAGCCAAGGAGCTNAGGATGCTGGAGCTTTTA
TCCCANCCTGCTGNCTTGCAGCTCCTGTTCACTCGNTNCGGGATCGCACACCTGACCAAAAACAAACNCGCTCATCCCC
CGCAGATTTTTNTGTTGCACTGCANNGGNCGAGGATGGGGCGGCCTGACCAGGTGACTGANCCTGGGGCAACACNCACT
CANCGACCCGNGGAGCTTGTATCACGGCGGAACACTACNTTGGTCTGGGCACCGGAATCCTTGGATGTTGCAGACTGCN
TGCGTTTNTCGTGGGCGCCTCTATCAGCTTTTCGTTTTTTTCCCTTCCACGCTCTGGTGGGGGCTTNNCCGCTCCNTTT
TCTTTCTTTTTTTTTCTTTTGCTCGGCACACTCCNCTCCGTGTGTTGTGTACGCGTTCCCGAGTTCGCACAAGTGTGAC
CCACGTTNGCNAGCGCCACAATTCCANAACAAGTTCTGTGTTTGNTGNGAAAAATNAAACAANNTCTTGGTTTCCACTT
GAAAACGAAAAGTAAAAGCGCGCCGCCAATTTG
so you can blast this yourself to confirm.
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