FB2025_01 , released February 20, 2025
Recombinant Construct: P{U6:2-ci.flySAM2.0}
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General Information
Symbol
P{U6:2-ci.flySAM2.0}
FlyBase ID
FBtp0132290
Feature type
transgenic_transposable_element
Component Allele(s)
cisgRNA.flySAM2.0
Size
Expression Data
Associated insertion(s)
0 available
Molecular map
Description and Uses
Construct components
Component
Use(s)
Marker(s)
MS2\MCPp65(h)::HSF1.10XUAS.flySAM2.0
Spyo\Cas9dCas9::VP64.10XUAS.flySAM2.0
Component allele cisgRNA.flySAM2.0
Transgenic product class
sgRNA
(Jia et al., 2018)
Component
Use(s)
Regulatory region(s)
snRNA:U6:96Ab
Encoded product / tool
ci
Tagged with
Tag:MS2
Description

The constitutive ' U6:2 ' (snRNA:U6:96Ab) promoter drives expression a single Tag:MS2-tagged sgRNA that forms one component of the three-component flySAM (synergistic activation mediator) system, which is used to produce effective transcriptional activation of a gene of interest. In this case, the sgRNA sequence (GCTATAACCACATACCCAAG) targets sequence upstream of ci.

(Jia et al., 2018)
Sequence Data
Sequence (FB)
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA Sequence
Extent
Segments and Size
Total Size
Left end
Right end
 
Segments
Num
Orientation
Symbol
Reference
Phenotypic Data
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Progenitors and Descendants
progenitor(s)
descendant(s)
Comments
Stocks (0)
Stocks containing insertion(s) of this recombinant construct
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
P{U6:2-ci.flySAM2.0}
(FlyBase, 1996-)
flySAM2.0-ci
(Jia et al., 2018)
Secondary FlyBase IDs
    References (2)