Allele Dmel\stnB¹⁴

General Information
Symbol	Dmel\stnB¹⁴	Species	D. melanogaster
Name		FlyBase ID	FBal0016214
Feature type	allele	Associated gene	Dmel\stnB
Also Known As	stn^PH1

Allele class	loss of function allele
Mutagen	P-element activity, PM hybrid dysgenesis
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Petrovich et al., 1993)
Mutagen	P-element activity PM hybrid dysgenesis (Zusman et al., 1985)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference An insertion of an I-element into the stnB ORF. (Kelly, 1997.1.23) Insertion into ORF2. (Andrews et al., 1996) I-element insertion. (Andrews et al., 1995)
Caused by insertion	I{}stnB¹⁴ (Andrews et al., 1996, Kelly, 1997.1.23, Andrews et al., 1995)
Cytology
Phenotypic Data
Phenotypic Class
	behavior defective \| recessive (Petrovich et al., 1993) lethal \| embryonic stage (Vijayakrishnan et al., 2010) lethal \| embryonic stage \| recessive (Fergestad et al., 1999) lethal \| recessive (Andrews et al., 1995, Petrovich et al., 1993) neurophysiology defective (Fergestad et al., 1999) uncoordinated \| embryonic stage \| recessive (Fergestad et al., 1999)
Phenotype Manifest In
	axon terminus (Fergestad et al., 1999) multivesicular body (Fergestad et al., 1999) synaptic vesicle (Fergestad et al., 1999)
Detailed Description
	Statement Reference stnA¹⁴ stnB¹⁴ "stn^PH1" mutant embryos show a more than 90% reduction in FM1-43 dye uptake at the neuromuscular junction after stimulus compared to wild type. (Fergestad and Broadie, 2001) Mutants die as mature embryos after a failure to hatch, due to lack of coordination. Mutant embryos show normal segmental patterning of the epidermis, muscles and nervous system. Mutant nmjs appear morphologically wild type though the nerve terminals are slightly smaller than normal. In electrophysiological recordings at the embryonic nmj nerve stimulation produces muscle contraction demonstrating that presynaptic depolarization evokes transmitter release and that muscle excitation-secretion response is intact. However evoked EJC peak amplitudes are significantly reduced and the release of neurotransmitter at mutant synapses is markedly asynchronous due to delayed presynaptic vesicle fusion. Mutants have an impaired ability to synchronously trigger calcium-mediated vesicle fusion. The overall level of neurotransmitter release is reduced. Variability in EJC peak amplitudes is increased, compared to wild type. Transmission fidelity is lost. Calcium sensitivity is unaltered. MEJC frequency is not significantly altered, though amplitude is increased, perhaps because of increased quantity of neurotransmitter in some vesicles. Mutant synapses exhibit severe fatigue after prolonged stimulation. Synapses show decreased synaptic vesicle density and accumulate membrane-recycling intermediates. (Fergestad et al., 1999) stnB¹⁴/Dp(1;Y)y⁺mal⁺ males are usually inviable, and survivors are typically sterile. (Stimson et al., 1998)
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference The introduction of stnB[14] into stmA[1]/stmA[rev499] animals does not cause detectable behavioral effects at permissive or restrictive rearing temperatures. (Vijayakrishnan et al., 2010)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	Gergen.
	Induced with: stnA¹⁴. (Fergestad et al., 1999)
Comments
	May be having effects on the expression from the stnA cistron. (Kelly, 1997.1.23) Possibly a null mutation, as judged from phenotypic comparisons with Df(1)30A. (Petrovich et al., 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	l(1)PH1 PH1 (Zusman et al., 1985) stn¹⁴ stn^PH1 (Fergestad and Broadie, 2001, Fergestad et al., 1999, Stimson et al., 1998, Kelly, 1997.1.23, Andrews et al., 1996, Andrews et al., 1995, Petrovich et al., 1993, Perrimon et al., 1989, Vijayakrishnan et al., 2010) stn^Ph1 (Kelly et al., 1987) stnB¹⁴
Name Synonym	stoned^PH1 (Vijayakrishnan et al., 2010)
Secondary FlyBase IDs

References ( 11 )
Research paper	Vijayakrishnan et al., 2010, Traffic 11(12): 1567--1578 Drosophila rolling blackout displays lipase domain-dependent and -independent endocytic functions downstream of dynamin. [FBrf0212267] Fergestad and Broadie, 2001, J. Neurosci. 21(4): 1218--1227 Interaction of Stoned and Synaptotagmin in synaptic vesicle endocytosis. [FBrf0134719] Fergestad et al., 1999, J. Neurosci. 19(14): 5847--5860 The stoned proteins regulate synaptic vesicle recycling in the presynaptic terminal. [FBrf0109701] Stimson et al., 1998, J. Neurosci. 18(23): 9638--9649 A product of the Drosophila stoned locus regulates neurotransmitter release. [FBrf0105332] Andrews et al., 1996, Genetics 143(4): 1699--1711 The stoned locus of Drosophila melanogaster produces a dicistronic transcript and encodes two distinct polypeptides. [FBrf0089585] Petrovich et al., 1993, Genetics 133(4): 955--965 A genetic analysis of the stoned locus and its interaction with dunce, shibire and Suppressor of stoned variants of Drosophila melanogaster. [FBrf0058577] Perrimon et al., 1989, Genetics 121: 313--331 Developmental genetics of loci at the base of the X chromosome of Drosophila melanogaster. [FBrf0049891] Kelly et al., 1987, J. Neurogenet. 4(2-3): 144--145 Characterization of stoned and suppressor of stoned mutants of Drosophila. [FBrf0046806] Zusman et al., 1985, D. I. S. 61: 217--218 Report of new mutants. [FBrf0042290]
Personal communication to FlyBase	Kelly, 1997.1.23, stoned is a dicistronic transcript. stoned is a dicistronic transcript. [FBrf0091346]
Abstract	Andrews et al., 1995, J. Neurogenet. 10(1): 16--17 Molecular analysis of the Drosophila stoned locus. [FBrf0079855]

Allele Dmel\stnB14

Allele Dmel\stnB¹⁴