Allele Dhyd\Minos\T^hs.PF

General Information
Symbol	Dhyd\Minos\T^hs.PF	Species	D. hydei
Name	heat shock construct of Franz	FlyBase ID	FBal0033602
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dhyd\Minos\T

Allele class
Mutagen	in vitro construct \| regulatory fusion, in vitro construct \| other
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Franz et al., 1994, Loukeris et al., 1995) in vitro construct \| other (Loukeris et al., 1995, Arca et al., 1997)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: Dhyd\Minos transposase gene is under the control of a heat shock promoter. (Franz et al., 1994) 456bp XbaI-XmnI fragment of Hsp70 promoter and 206bp of untranslated leader sequence replace the 5' inverted repeat of Dhyd\Minos\T. (Loukeris et al., 1995) Hsp70 promoter drives expresson of Dhyd\Minos\T missing the 5' terminus. (Loukeris et al., 1995) Left hand repeat of Dhyd\Minos is replaced by the Hsp70 promoter. (Arca et al., 1997) An Hsp70 promoter drives expression of an intronless Dhyd\Minos\T^hs.PF gene. (Metaxakis et al., 2005)
Assay mode	Whole-organism transient assay (allele from one drosophilid species assayed in another drosophilid) (Metaxakis et al., 2005, Loukeris et al., 1995) In transgenic Drosophila (allele of one drosophilid species in genome of another drosophilid) (Franz et al., 1994, Arca et al., 1997, Loukeris et al., 1995)
Carried in construct	P{hsMi2} (Franz et al., 1994, Loukeris et al., 1995, Arca et al., 1997) P{hsILMiT} (Metaxakis et al., 2005)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	24613 w¹¹¹⁸; noc^Sco/SM6a, P{hsILMiT}2.4
Notes on Origin
Discoverer

Comments
	Used as a Dhyd\Minos transposase source assayed in the D.melanogaster genome. (Loukeris et al., 1995) Carried in plasmid pHSS6hsMi2, expressed in D.melanogaster flies as a source of transposase. (Loukeris et al., 1995) A defective Dhyd\Minos transposon that is inserted into the D.melanogaster genome is stable in the absence of Dhyd\Minos\T^hs.PF but is mobilised at relatively high frequencies in the presence of transposase. (Arca et al., 1997) Carried in plasmid pHSS6hsMi2 and injected into D.melanogaster preblastoderm embryos as a source of transposase. Also carried in P{hsILMiT} and used to generate transgenic D.melanogaster flies containing a helper chromosome providing transposase. (Metaxakis et al., 2005)
Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Dhyd\Minos\T^hs.PF
Name Synonym	heat shock construct of Franz
Secondary FlyBase IDs

References ( 6 )
Research paper	Metaxakis et al., 2005, Genetics 171(2): 571--581 Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750] Arca et al., 1997, Genetics 145(2): 267--279 Mobilization of a Minos transposon in Drosophila melanogaster chromosomes and chromatid repair by heteroduplex formation. [FBrf0092450] Loukeris et al., 1995, Proc. Natl. Acad. Sci. USA 92(21): 9485--9489 Introduction of the transposable element Minos into the germ line of Drosophila melanogaster. [FBrf0084149] Loukeris et al., 1995, Science 270(5244): 2002--2005 Gene transfer into the medfly, Ceratitis capitata, with a Drosophila hydei transposable element. [FBrf0085479] Franz et al., 1994, Proc. Natl. Acad. Sci. USA 91(11): 4746--4750 Mobile Minos elements from Drosophila hydei encode a two-exon transposase with similarity to the paired DNA-binding domain. [FBrf0073095]
Abstract	Savakis et al., 1995, A. Dros. Res. Conf. 36: 208A Transposition of the Minos element in D. melanogaster. [FBrf0079329]

Allele Dhyd\Minos\Ths.PF

Allele Dhyd\Minos\T^hs.PF