Allele Dhyd\Minos\T^hs.PF

General Information
Symbol	Dhyd\Minos\T^hs.PF	Species	D. hydei
Name	heat shock construct of Franz	FlyBase ID	FBal0033602
Feature type	allele	Associated gene	Dhyd\Minos\T

Allele class
Mutagen	in vitro construct - regulatory fusion, in vitro construct
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct (Loukeris et al., 1995, Arca et al., 1997) in vitro construct - regulatory fusion (Franz et al., 1994, Loukeris et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference An Hsp70 promoter drives expression of an intronless Dhyd\Minos\T^hs.PF gene. (Metaxakis et al., 2005) Left hand repeat of Dhyd\Minos is replaced by the Hsp70 promoter. (Arca et al., 1997) 456bp XbaI-XmnI fragment of Hsp70 promoter and 206bp of untranslated leader sequence replace the 5' inverted repeat of Dhyd\Minos\T. (Loukeris et al., 1995) Hsp70 promoter drives expresson of Dhyd\Minos\T missing the 5' terminus. (Loukeris et al., 1995) Construct: Dhyd\Minos transposase gene is under the control of a heat shock promoter. (Franz et al., 1994)
Carried in construct	P{hsILMiT} (Metaxakis et al., 2005) P{hsMi2} (Franz et al., 1994, Loukeris et al., 1995, Arca et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	24613 w¹¹¹⁸; sna^Sco/SM6a, P{hsILMiT}2.4 36311 y¹ w^*; sna^Sco/SM6a, P{hsILMiT}2.4
Notes on Origin
Discoverer

Comments
	Carried in plasmid pHSS6hsMi2 and injected into D.melanogaster preblastoderm embryos as a source of transposase. Also carried in P{hsILMiT} and used to generate transgenic D.melanogaster flies containing a helper chromosome providing transposase. (Metaxakis et al., 2005) A defective Dhyd\Minos transposon that is inserted into the D.melanogaster genome is stable in the absence of Dhyd\Minos\T^hs.PF but is mobilised at relatively high frequencies in the presence of transposase. (Arca et al., 1997) Used as a Dhyd\Minos transposase source assayed in the D.melanogaster genome. (Loukeris et al., 1995) Carried in plasmid pHSS6hsMi2, expressed in D.melanogaster flies as a source of transposase. (Loukeris et al., 1995)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Dhyd\Minos\T^hs.PF
Name Synonym	heat shock construct of Franz
Secondary FlyBase IDs

References ( 6 )
Research paper	Metaxakis et al., 2005, Genetics 171(2): 571--581 Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750] Arca et al., 1997, Genetics 145(2): 267--279 Mobilization of a Minos transposon in Drosophila melanogaster chromosomes and chromatid repair by heteroduplex formation. [FBrf0092450] Loukeris et al., 1995, Proc. Natl. Acad. Sci. U.S.A. 92(21): 9485--9489 Introduction of the transposable element Minos into the germ line of Drosophila melanogaster. [FBrf0084149] Loukeris et al., 1995, Science 270(5244): 2002--2005 Gene transfer into the medfly, Ceratitis capitata, with a Drosophila hydei transposable element. [FBrf0085479] Franz et al., 1994, Proc. Natl. Acad. Sci. U.S.A. 91(11): 4746--4750 Mobile Minos elements from Drosophila hydei encode a two-exon transposase with similarity to the paired DNA-binding domain. [FBrf0073095]
Abstract	Savakis et al., 1995, A. Dros. Res. Conf. 36: 208A Transposition of the Minos element in D. melanogaster. [FBrf0079329]

Allele Dhyd\Minos\Ths.PF

Allele Dhyd\Minos\T^hs.PF