A Database of Drosophila Genes & Genomes

FB2008_07, released August 8, 2008
 

Allele Dhyd\Minos\Ths.PF

General Information
SymbolDhyd\Minos\Ths.PFSpeciesD. hydei
Nameheat shock construct of FranzFlyBase IDFBal0033602
Feature typealleleCreated / Updated2006-08-20/2006-08-20
Associated geneDhyd\Minos\T
Allele class
Mutagenin vitro construct | regulatory fusionin vitro construct | other
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Allele class
Mutagen
Mapped Features and Mutations
Type
Symbol & Location
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References
 
 
 
 
Associated Sequence Data
DDBJ /
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GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Progenitor genotype
      Nature of the lesion
      Statement
      Reference
      Construct: Dhyd\Minos transposase gene is under the control of a heat shock promoter.
      456bp XbaI-XmnI fragment of Hsp70 promoter and 206bp of untranslated leader sequence replace the 5' inverted repeat of Dhyd\Minos\T.
      Hsp70 promoter drives expresson of Dhyd\Minos\T missing the 5' terminus.
      Left hand repeat of Dhyd\Minos is replaced by the Hsp70 promoter.
      An Hsp70 promoter drives expression of an intronless Dhyd\Minos\Ths.PF gene.
      Assay mode
      Carried in construct
      Cytology
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      Comments
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      Bloomington
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      Discoverer
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      Used as a Dhyd\Minos transposase source assayed in the D.melanogaster genome.
      Carried in plasmid pHSS6hsMi2, expressed in D.melanogaster flies as a source of transposase.
      A defective Dhyd\Minos transposon that is inserted into the D.melanogaster genome is stable in the absence of Dhyd\Minos\Ths.PF but is mobilised at relatively high frequencies in the presence of transposase.
      Carried in plasmid pHSS6hsMi2 and injected into D.melanogaster preblastoderm embryos as a source of transposase. Also carried in P{hsILMiT} and used to generate transgenic D.melanogaster flies containing a helper chromosome providing transposase.
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      Reported As
      Symbol Synonym
      Dhyd\Minos\Ths.PF
       
      Name Synonym
      heat shock construct of Franz
       
      Secondary FlyBase IDs
        hide References ( 6 )
        Research paper
        Metaxakis et al., 2005, Genetics 171(2): 571--581
        Minos as a genetic and genomic tool in Drosophila melanogaster. [FBrf0190750]
        Arca et al., 1997, Genetics 145(2): 267--279
        Mobilization of a Minos transposon in Drosophila melanogaster chromosomes and chromatid repair by heteroduplex formation. [FBrf0092450]
        Loukeris et al., 1995, Proc. Natl. Acad. Sci. USA 92(21): 9485--9489
        Introduction of the transposable element Minos into the germ line of Drosophila melanogaster. [FBrf0084149]
        Loukeris et al., 1995, Science 270(5244): 2002--2005
        Gene transfer into the medfly, Ceratitis capitata, with a Drosophila hydei transposable element. [FBrf0085479]
        Franz et al., 1994, Proc. Natl. Acad. Sci. USA 91(11): 4746--4750
        Mobile Minos elements from Drosophila hydei encode a two-exon transposase with similarity to the paired DNA-binding domain. [FBrf0073095]
        Abstract
        Savakis et al., 1995, A. Dros. Res. Conf. 36: 208A
        Transposition of the Minos element in D. melanogaster. [FBrf0079329]