FB2025_01 , released February 20, 2025
Allele: Ecol\lacZsli.1.0HV
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General Information
Symbol
Ecol\lacZsli.1.0HV
Species
E. coli
Name
FlyBase ID
FBal0056589
Feature type
allele
Associated gene
Ecol\lacZ
Associated Insertion(s)
Carried in Construct
P{sli(1.0)-lacZ}
Also Known As
P[1.0slit-lacZ], P[sli 1.0 HV-lacZ], Psli-1.0-lacZ
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Wharton and Crews, 1993, Nambu et al., 1991)
Progenitor genotype
Carried in construct
P{sli(1.0)-lacZ}
Cytology
Description

1.0kb HindIII-EcoRV fragment of sli intron sequence is fused, in reverse orientation, upstream of the P\T promoter (from -48 relative to the transcription start site) and N-terminal P\T sequence fused in frame to lacZ. The N-terminal P\T sequence includes the Tag:NLS(P) nuclear localization signal.

(Wharton and Crews, 1993)
Allele components
Component
Use(s)
Regulatory region(s)
sli_1.0_HV
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      distribution deduced from reporter
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage 11 -- 17
      medial cell body glial cell
      (Zhou et al., 1997)
      embryonic stage 13 -- 17
      midline glial cell
      (Bergmann et al., 2002)
      embryonic stage 15
      midline glial cell
      (Ma et al., 2000)
      Additional Information
      Statement
      Reference

      This reporter construct drives lacZ expression in the midline glial cells of the developing embryonic nervous system.

      (Bergmann et al., 2002)
       
      Marker for
      midline glial cell
      (Bergmann et al., 2002)
      Reflects expression of
      sli
      (Bergmann et al., 2002, Ma et al., 2000)
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Ecol\lacZsli.1.0HV
      lacZsli.1.0HV
      lacZsli.P1.0
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0041955
      References (24)