FlyBase Allele Report: Dmel\Top3β[26]

General Information

Symbol

Dmel\Top3β²⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0195411

Feature type

allele

Associated gene

Dmel\Top3β

Associated Insertion(s)

P{?EP}Top3β²⁶

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Wu et al., 2006)

Mutagen

P-element activity

(Wu et al., 2006)

Progenitor genotype

P{EP}Top3β^EP1432

(Wu et al., 2006)

Associated Insertion(s)

P{?EP}Top3β²⁶

Cytology

Description

Imprecise excision of the P{EP} element, resulting in a 400bp deletion in the second exon of Top3β, from nucleotide 495 to 894 downstream from the translation start site. There is a 170bp fragment of the P{EP} element left behind.

(Wu et al., 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Xu et al., 2013)

abnormal neuroanatomy | third instar larval stage (with Top3β¹⁶)

(Ahmad et al., 2017)

fertile

(Wu et al., 2006)

viable

(Wu et al., 2006)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage

(Xu et al., 2013)

embryonic/larval neuromuscular junction | third instar larval stage (with Top3β¹⁶)

(Ahmad et al., 2017)

NMJ bouton | third instar larval stage

(Xu et al., 2013)

NMJ bouton | third instar larval stage (with Top3β¹⁶)

(Ahmad et al., 2017)

Detailed Description

Statement

Reference

The third instar larval neuromuscular junction of Top3β²⁶/Top3β¹⁶ transheterozygotes exhibits significantly more branches and boutons than controls.

(Ahmad et al., 2017)

Top3β²⁶ mutant flies have normal smooth eyes with no necrotic ommatidia.

Top3β²⁶ mutant third instar larvae exhibit neuromuscular junction abnormalities in muscle 4. Their synaptic branch and bouton numbers are significantly higher than in wild type flies.

(Xu et al., 2013)

Top3β²⁶ mutants show no pronounced fertility defects and their lifespan is normal.

(Wu et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Top3β²⁶ has abnormal neuroanatomy | third instar larval stage phenotype, suppressible | partially by Fmr1^Δ50M

(Xu et al., 2013)

Enhancer of

Statement

Reference

Top3β²⁶ is an enhancer of visible phenotype of Fmr1^sev.PW

(Xu et al., 2013)

Suppressor of

Statement

Reference

Top3β²⁶ is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Fmr1^Δ50M

(Xu et al., 2013)

Phenotype Manifest In

Suppressed by

Statement

Reference

Top3β²⁶ has embryonic/larval neuromuscular junction | third instar larval stage phenotype, suppressible | partially by Fmr1^Δ50M

(Xu et al., 2013)

Top3β²⁶ has NMJ bouton | third instar larval stage phenotype, suppressible | partially by Fmr1^Δ50M

(Xu et al., 2013)

Enhancer of

Statement

Reference

Top3β²⁶ is an enhancer of eye phenotype of Fmr1^sev.PW

(Xu et al., 2013)

Top3β²⁶ is an enhancer of ommatidium phenotype of Fmr1^sev.PW

(Xu et al., 2013)

Suppressor of

Statement

Reference

Top3β²⁶ is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Fmr1^Δ50M

(Xu et al., 2013)

Top3β²⁶ is a suppressor of NMJ bouton | third instar larval stage phenotype of Fmr1^Δ50M

(Xu et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The number of synaptic branches and boutons in muscle 4 neuromuscular junctions is significantly lower in Top3β²⁶ Fmr1^Δ50M double mutant third instar larvae than in either mutant alone.

A Top3β²⁶ mutant background enhances the rough eye phenotype seen in flies expressing Fmr1^sev.PW. Flies show a threefold increase in necrosis and a more disorganised photoreceptor cluster in each ommatidium when compared to expression of Fmr1^sev.PW in a wild type background.

(Xu et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Top3β²⁶/Top3β¹⁶ is partially rescued by Scer\GAL4^elav.PU/Top3β^{WT.UAS.Tag:FLAG}

(Ahmad et al., 2017)

Not rescued by

Top3β²⁶/Top3β¹⁶ is not rescued by Scer\GAL4^elav.PU/Top3β^{ΔRGG.UAS.Tag:FLAG}

(Ahmad et al., 2017)

Top3β²⁶/Top3β¹⁶ is not rescued by Scer\GAL4^elav.PU/Top3β^{Y322F.UAS.Tag:FLAG}

(Ahmad et al., 2017)

Top3β²⁶/Top3β¹⁶ is not rescued by Top3β^{C660R.UAS.Tag:FLAG}/Scer\GAL4^elav.PU

(Ahmad et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Top3β²⁶

(Jauregui-Lozano et al., 2022, Lee et al., 2018)

Top3β^KO

(Ahmad et al., 2017)

top3β²⁶

(Wu et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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