Inactivation of P-Fr neutrons by the expression of Ctet\tetX^TNT-LC.UAS under the control of Scer\GAL4^GMR37G12 leads to resistance to sleep deprivation and a higher arousal threshold at night time while it does not lead to any changes in the sleep/wake cycles, total duration of night time sleep, locomotion (except during nighttime, which leads to a significant increase in sleep) or mechanosensory responsiveness of flies when compared to control adults.

Optogenetic activation of P-Fr neurons via expression of Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} under the control of Scer\GAL4^GMR37G12 leads to a lower arousal threshold at night time when compared to control adults.

Thermogenetic activation of P-Fr neurons via expression of TrpA1^UAS.(B).cKa under the control of Scer\GAL4^GMR37G12 leads to significantly less sleep at night time, a lower arousal threshold at night time, and a significant increase in locomotion of flies during daytime with no change during nighttime when compared to control adults.