FB2025_01 , released February 20, 2025
Allele: Dmel\Pif1AGFP.FPTB
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General Information
Symbol
Dmel\Pif1AGFP.FPTB
Species
D. melanogaster
Name
FlyBase ID
FBal0280788
Feature type
allele
Associated gene
Dmel\Pif1A
Associated Insertion(s)
Carried in Construct
PBac{Pif1A-GFP.FPTB}
Key Links
Transgenic product class
genomic_DNA
(Spokony, 2012.12.12)
wild_type
(Spokony, 2012.12.12)
Mutagen
Nature of the Allele
Transgenic product class
genomic_DNA
(Spokony, 2012.12.12)
wild_type
(Spokony, 2012.12.12)
Mutagen
in vitro construct
(Spokony, 2012.12.12)
Progenitor genotype
Carried in construct
PBac{Pif1A-GFP.FPTB}
Cytology
Description

Genomic fragment including the Pif1A gene, from BAC clone CH321-37J05. A sfGFP-Tag:FLAG-Tag:CS(PSP)-Tag:CS(TEVp)-Tag:BLRP tag cassette has been introduced at the C-terminal end of the Pif1A gene, such that protein isoform I is tagged.

(Spokony, 2012.12.12)
Allele components
Component
Use(s)
Regulatory region(s)
Pif1A
Encoded product / tool
Pif1A
Tagged with
sfGFP
Tag:BLRP
Tag:CS(PSP)
Tag:CS(TEVp)
Tag:FLAG
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Pif1AGFP.FPTB rescues Pif1A1

      (Yuan et al., 2019)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Pif1AGFP.FPTB
      Pif1AT:Avic\GFP-SF,T:Zzzz\FLAG,T:Zzzz\PSP.CS,T:Zzzz\TEV.CS,T:Zzzz\BLRP
      Pif1AsfGFP,Tag:FLAG,Tag:CS(PSP),Tag:CS(TEVp),Tag:BLRP
      Name Synonyms
      Secondary FlyBase IDs
        References (3)