FB2025_01 , released February 20, 2025
Allele: Disc\RFPmCherry.nanos.Tag:MCP,Tag:FLAG,Tag:NLS(SV40-largeT)
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General Information
Symbol
Disc\RFPmCherry.nanos.Tag:MCP,Tag:FLAG,Tag:NLS(SV40-largeT)
Species
D. sp.
Name
FlyBase ID
FBal0340434
Feature type
allele
Associated gene
Disc\RFP
Associated Insertion(s)
Carried in Construct
P{nanos-MCP-mCherry-FLAG}
Transgenic product class
Mutagen
Nature of the Allele
Transgenic product class
Mutagen
in vitro construct
(Hayashi et al., 2014)
Progenitor genotype
Carried in construct
P{nanos-MCP-mCherry-FLAG}
Cytology
Description

nos promoter sequences drive expression of a Tag:MCP-mCherry fusion protein. A Tag:NLS(SV40-largeT) nuclear localization signal is present at the N-terminal end of the open reading frame (ORF) and a Tag:FLAG epitope is present at the C-terminal end of the ORF. The portion of the fs(1)K10 3'UTR which stabilises germline transcripts and restricts them to nurse cells is also present. FlyBase curator comment: in the presence of a mRNA tagged with Tag:MS2 (an RNA stem loop to which Tag:MCP binds), the Tag:MCP-mCherry fusion protein can bind to this tagged mRNA, allowing its localization within the cell to be studied. In the absence of a Tag:MS2-tagged mRNA (or when unbound), the Tag:MCP-mCherry fusion protein will be targeted to the nucleus due to the presence of the Tag:NLS(SV40-largeT) tag.

(Hayashi et al., 2014)
Allele components
Component
Use(s)
Regulatory region(s)
nanos
Encoded product / tool
mCherry
Tagged with
Tag:FLAG
Tag:MCP
Tag:NLS(SV40-largeT)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (6)
      Reported As
      Symbol Synonym
      Disc\RFPmCherry.nanos.Tag:MCP,Tag:FLAG,Tag:NLS(SV40-largeT)
      Disc\RFPmCherry.nos.Tag:MCP,Tag:FLAG,Tag:NLS(SV40-largeT)
      Disc\RFPmCherry.nos.Tag:MS2-CP,Tag:FLAG,Tag:NLS(SV40-largeT)
      Disc\RFPmCherry.nos.Tag:MS2-CP,Tag:FLAG
      MS2\MCPnos.T:Disc\RFP-mCherry,T:Zzzz\FLAG
      MS2\MCPnos.mCherry,Tag:FLAG
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0296206
      • FBal0340402
      References (2)