A TI{CRIMIC.GT14} DNA cassette has been inserted into Atg8a, in a coding intron of 2 of 3 annotated transcripts of the gene (insertion is in the 5'UTR of the other transcript), and is predicted to gene trap the gene. The TI{CRIMIC.GT14} cassette was inserted using the CRISPR/Cas9 technique together with a donor plasmid to drive homology directed repair. The sgRNA sequence used to target the gene was: CTAGACGATCCGTTCTAGCCAGG. The homology arms of the donor plasmid used were designed such that there is a small gap between the 3' end of the 5' arm and the 5' end of the 3' arm, thus the insertion of the TI{CRIMIC.GT14} cassette is predicted to be accompanied by a deletion of 11bp of genomic sequence flanking the insertion site.