FB2025_01 , released February 20, 2025
Allele: Dmel\Sap47MI09900-DH.PT-GFSTF.2
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General Information
Symbol
Dmel\Sap47MI09900-DH.PT-GFSTF.2
Species
D. melanogaster
Name
FlyBase ID
FBal0341627
Feature type
allele
Associated gene
Dmel\Sap47
Associated Insertion(s)
Mi{DH.2}Sap47MI09900-DH.PT-GFSTF.2
Carried in Construct
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
phiC31 integrase
(Li-Kroeger et al., 2018)
Progenitor genotype
Mi{MIC}Sap47MI09900
(Li-Kroeger et al., 2018)
Associated Insertion(s)
Mi{DH.2}Sap47MI09900-DH.PT-GFSTF.2
Cytology
Description

The RMCE cassette in the Mi{MIC}Sap47MI09900 insertion has been replaced with a 'Double Header' (DH) trapping cassette (contains two independent trapping modules oriented in opposite directions). The cassette is in 'protein-trap' orientation relative to Sap47, which is predicted to result in the Sap47 coding sequence being tagged with a EGFP-Tag:CALI(TC)-Tag:StrepII-Tag:CS(TEVp)-3xTag:FLAG multi-tag cassette.

(Li-Kroeger et al., 2018)
Allele components
Component
Use(s)
Encoded product / tool
Tagged with
EGFP
Tag:CALI(TC)
Tag:CS(TEVp)
Tag:FLAG
Tag:StrepII
Mutations Mapped to the Genome
Curation Data
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Sap47MI09900-DH.PT-EGFP.2
      Sap47MI09900-DH.PT-GFSTF.2
      Name Synonyms
      Secondary FlyBase IDs
        References (2)