FB2025_01 , released February 20, 2025
Allele: Dmel\kelORF2-FLAG,NLS::tdGFP
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General Information
Symbol
Dmel\kelORF2-FLAG,NLS::tdGFP
Species
D. melanogaster
Name
FlyBase ID
FBal0367692
Feature type
allele
Associated gene
Dmel\kel
Associated Insertion(s)
TI{TI}kelORF2-FLAG,NLS::tdGFP
Carried in Construct
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Hudson et al., 2021)
gene targeting by homologous recombination
(Hudson et al., 2021)
Progenitor genotype
Associated Insertion(s)
TI{TI}kelORF2-FLAG,NLS::tdGFP
Cytology
Description

A readthrough reporter cassette has been inserted immediately 5' of the stop codon of ORF2 in the endogenous kel locus. The reporter cassette consists of three copies of the Tag:FLAG epitope, a T2A sequence and a ' NLS::tdGFP ' open reading frame (encodes nuclear-localized tandem dimer GFP). The reporter cassette allows for specific detection of the readthrough product using either the Tag:FLAG epitopes at the C-terminus of ORF2, or detecting the presence of the NLS::tdGFP protein (the presence of the T2A sequence results in the NLS::tdGFP marker being produced as a separate protein following readthrough translation).

(Hudson et al., 2021)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      kelORF2-FLAG,NLS::tdGFP
      Name Synonyms
      Secondary FlyBase IDs
        References (1)