FB2025_01 , released February 20, 2025
Allele: Avic\GFPLkr-CCT1035C
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General Information
Symbol
Avic\GFPLkr-CCT1035C
Species
A. victoria
Name
FlyBase ID
FBal0402884
Feature type
allele
Associated gene
Avic\GFP
Associated Insertion(s)
TI{2A-EGFP}LkrCCT1035C
Carried in Construct
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
CRISPR/Cas9
(Mao et al., 2024)
gene targeting by homologous recombination
(Mao et al., 2024)
Progenitor genotype
TI{TI}LkrattP
(Mao et al., 2024)
Associated Insertion(s)
TI{2A-EGFP}LkrCCT1035C
Cytology
Description

A sequence cassette has been introduced into the attP site present in TI{TI}LkrattP, restoring the deleted Lkr sequence and inserting a 2A-EGFP sequence at the C-terminal end of the open reading frame. In addition, two FRT sites have been inserted, one at the N-terminal end of the reintegrated sequence, and the other downstream of the 2A-EGFP sequence (a single loxP site is also present, downstream of the second FRT site). This results in a conditional allele; FLP recombinase can be used to excise the artificial FRT cassette, excising the 2A-EGFP sequence and removing Lkr coding sequence, disrupting its function.

(Mao et al., 2024)
Allele components
Component
Use(s)
Inserted element
TI{2A-EGFP}
Encoded product / tool
EGFP
Also carries
FRT
loxP
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Avic\GFPLkr-CCT1035C
      Name Synonyms
      Secondary FlyBase IDs
        References (1)