FB2025_01 , released February 20, 2025
Clone: Dmel\AF083321
Open Close
General Information
Symbol
Dmel\AF083321
Species
D. melanogaster
Name
AF083321
FlyBase ID
FBcl0000589
Feature type
cDNA_clone
Computed gene(s)
Dmel\RpS27
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    AF_cDNA
    • AF cDNA library of D. melanogaster, Canton-S strain, adult brain.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      adult stage
      adult brain
      (Posey et al., 2001)
      Sequence Data of the Insert
      Partial Sequence
      Total bases
      438
      NCBI
      AF083321
      Partial sequence dataSequence Downloader
      AGAAAAAACATGCCGCTAGCAAAAGATCTTCTGCTCCCTCTGCCCGCCGAGGAGAAGCGCAAGCACAAGCTGAAGCGCCT
GGTCCAGCACCCCAACTCGTACTTCATGGACGTGAAGTGCCCCGGCTGCTACAGGATCACCACCGTCTTCAGCCACGCCC
AAGGCGTCGTGGTCTGCGCTGGATGCGCTACCATTTTGTGCCAGCCGACTGGAGGACGCGCCAAGCTGACAGAAGGCTGC
TCCTTCCGCAGGAAGCCACAGTAAAATGGGACTCGCGATGAACCACAAATTATTAATTTTTTAATAAAAACTCTAAAACG
TAGAGAAACCACAGAAACCCATACGAGAGAAAGCTTGTTATTCAATTGCTGCGGTCCTTTGGTTCATTGTGCTTTGTGAA
TTAAAGAATTAACGATGTTGTGGGTCNGCTTAGTGAAA
      Library Information (1)
      Library: AF_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from adult brains. The strain used was not the iso-1 sequenced strain.

      (Posey et al., 2001)
      Sample preparation

      Tissue was dessicated using the Stratagene HybriZAP system.

      Protocol

      Library constructed from dessicated tissue using the Stratagene HybriZAP system. Library was designed for protein expression, and constructed such that full-length cDNAs containing 5' untranslated regions are unlikely to be present.

      (Posey et al., 2001)
      Comment

      GenBank AF083295-AF083548, AF171701-AF171760.

      (Posey et al., 2001)
      Reported As
      Symbol Synonyms
      AF
      AF_cDNA
      References (1)
      Research paper (1)
      Posey et al., 2001, Genome Biol. 2(3): RESEARCH0008
      Survey of transcripts in the adult Drosophila brain. [FBrf0135825]
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)