ATTTTTTGGACCGTAAAGTGCAAAATTTTCATATGATCTGTGTCAAAAGAATGCCGTGGAGCAATGTTCGCATATTTCTG AAGCTCGCTTCCCAGCGATGCCAGCTCCTAAGTGTGGGCTCCATCCAAGAGGCGTCTGCAGGACACCGATCGGGCTCCTT CGAATCGGATGCCCTGTCGAGCACTTCGAATGCCAGGCACATGGAGTGCCTGTTCGCCAAGTGGCTGGGGGACACTAGCT CGGTCAATGGAACCTGGCAGAATTTCTTCAAGGGAATGGTAGAGAAACAAATAGACTCGCAACCGCCTCGGAATAAAGAA GCAGAAGGAGATGTTCTTCCCTCGATAGGAACCACAACGCCAACAGATGCAGCGATTGGGGCTCCAGTTTTGGGAAGGAG AAAAGCAGTCTCTTCTTCGGCACAGCAGAGTCCTTTGGACACTTCGGAAGCGGACTTCAACAGTTGCGTGGCTCAGTCTC AAAAGCATGATATTGGAGTGCTTTCACACTCAACGGATATCAGAACTTCTGGCATATACGACAAACAGAAACTGCCCACA TTGCCCAAAGATGGCATTCTGAAAGGCTGTTCAATAAACGGATCAAATATAGCCAGTGAAGCTCATTCATCGATTTTATT GGGGAATGAAGTGGCACACAACATTTCCAAAAACTTGACCAGAAAAATGTCGCAAAAAAATACTCCAGACAAGCCAGAAA AACCACATAAGCATGAAGAAATCTTGACGGGTTCCAGAAGAACCGGAAACTCGCGCAACAACTTTCAAAAGAAATTCGGC TCATTCGAAATTTTTATGAAATTCTGGAAACACGCACATGCATTGGATAAGTATAAGCGGCATATTGGTCCAGTAATGGT TGTGAAGAAGCGTTCAAAAACCAATTCAAAGCCAATTAACGTCATGTACTTCAAAAATTATTACGAAAAGGGTTTACGTC TAAGGAAGGAAAGGTTGAATAAAATCAAAAGCAATAAGAAAATTAAAATGCCTTCAAAAAGCCCTAAGGAGTCCGTAGCT AAAGAAATCATTGAAAATGCCAATATACCACAATCTCACCCAGAAACAAAGAAATCCACATATGTTTCAAAACACAAGAG CGATACATATGCGACCCTTGAGGAACTGATCAACGATATTGATGACATCGCCATAAATGAGAAGTTGTCTGAATCGATTG AGCCAGTGAAAACCAAGAAAGACAGTAAAACACCGAAGCTGTTGGAGAACACTCCCAAAAAGAAGCCAAAGAATTTCAAA ACGGCACTTAGGGTTTTCAAGCCCGAAGAGAAGGTTATCCCAAAATCGGATAATATGCCGAGCTCAAATATCAAGCCCAA TCCCGTTTCCAAGTTGGACAAATCCAAATTAAGTAAGCCGAAGACTGAAAAATACATTCCTTTGAGGGTTTTCAATACAC CAAAAGAAGACGAAGACAAGTCATGGATATATTGGGATGAGTTGGCAGTGAAAAGCCTGCGAAAACCAACTCAAGACTAG CACTTCATCCTCAGAAACCCAAGAAGGAAGTTGGGCAGCCTCTTCATCCTTTGAACTGAAACTAACCTAACGAATCTCAG CACGTCTTGCCACAAGTGATTCCTACTATGAGCTGATCTCACAACTTTCTCAGCCTTCGAACCCTAAGCACCAAATTCAA AATTTAACAGGTATTTCGGTAGTCCCTTCTAGAACATGAACCAATGTCGTCTGAGGAGCCTGGCGCGGATCAGGAGGAGC CTGACACTGGGTTTGCGGGGAACTGACCAACACGTCCTCGCCCGTCAAGCCCTCCGCACCATTCAAACGACCTCACAGCG GCGAGGTGTCCATGATCTGGACTCCTTCGCCAATGGATGCAGTGCCGCCTACATCGAGGGACTCTACAACAAGTGGAAGC GCAATCCCAGTTCCGTAGATGAGTCCTGGAACGAGCTCTTCAGCAGCAACGATTGGTCCAGCCCCAAGAGAAGTCCTCTT CAGGTTTCGCACTCCCGGAAGTACAGACGTCCTCCGGTTGAAAGGATAGCTGTGAAGGCTCGGTCGGGCGAAAGGACGGC TTCCGGTGGCGCATCTGCAGCTCCAGCCGCACCTCCCAGCGACTGGAAGAACATTGATGACCACCACGTGATCCAGGCCA TCATCCGTGCCTATCAATCGCGCGGTCACTTGGCCGCCGATCTGGATCCCTTGGGTATTGTTGGTCCGAAGAAGCGTACG TCGGTGGATGGCACCCAGCGACATGCCGCCAGGGAGGTGCTCCGCCAGCACTTCTCCTATATATTTAATGACCTCAATAC CGTGTTCAAGTTGCCCTCATCCACAATGATAGGCGGTGATCAGGAATTTCTGTCACTAAAAGAGATCCTGGATCGCTTGG AGCGCATCTATTGCGGCCACATTGGAGTGGAGTACATGCAGATCACGTCGTTGACAAAGACCAACTGGCTTCGCGACCGT TTCGAGAAGCCTGGAGGCCTGGACTTGACCAAGGAGGAAAAGAAGCTCATCCTAGAGCGTCTCACTCGCTCGACGGGATT CGAAAACTTCCTGGCGAAAAAGTTCTCGTCGGAGAAGCGTTTCGGACTGGAGGGATGCGACATTATGATACCCGCTATAA AGGAGGTGGTTGACCGGGCCACGGACCATGGCGTGGAGTCAATTTTAATAGGAATGGCGCATCGAGGGCGCCTCAATGTC CTCGCCAACATCTGTCGCAAGCCCATTTCGGACATCCTGTCTCAGTTCCACGGCTTGCAGGCCACCGATTCCGGATCTGG CGACGTTAAGTACCATCTGGGCGTGTTTCAAGAGCGGCTCAACCGACAGACAAACCGCATGGTGCGCATCACCGTGGTGG CGAATCCCTCTCACTTGGAGCACGTCAACCCGGTGCTCCTGGGTAAGGCCCGAGCGGAGATGTTCCAACGGGGCGACACA TGTGGCAGCACGGTGATGCCCATCATTATCCACGGAGATGCCTCCTTTTCCGGTCAGGGCGTGGTCTACGAATCAATGCA TCTGTCCGACTTGCCCAACTACACCACCTACGGCACCATTCACATTGTGTCCAACAACCAGGTGGGCTTCACGACAGATC CGCGTTTCTCCCGCTCCTCGCGCTACTGCACGGACGTTGCCAAGGTGGTGAATGCTCCGATACTGCACGTGAATGCGGAC GATCCGGAGGCGTGCATCCAGTGCGCTCGCATTGCCATCGACTATCGGACACGATTCAAGAAGGATGTGGTCATCGACAT AGTTGGCTACCGACGCAACGGGCACAATGAGGCGGATGAACCCATGTTCACCCAGCCGCTGATGTACCAGCGCATAAAAA AACTGAAGCCATGCCTCCAGCTCTACGCCGATAAGTTGATCAAGGAGGGAGTCGTCACGGATAGCGAGTTCAAGGCAATG GTGTCAAGCTACGAAAAGATCTGCGAGGACGCTTGGGCCAAGTCCAAGACCATCAAGACCATAAAGTATTCCTCTTGGAT CGACTCTCCCTGGCCAGGATTCTTCGAGGGTCGCGATCGCCTGAAACTGTGCCCCACTGGCATCAGCACGGACACCCTGA AGACGATTGGCAACATGTTCTCGACTCCTCCGCCGCCGGAGCACAAGTTCGAGACACACAAGGGCATCCTTCGCATACTG GCCCAGCGCACGCAGATGGTGCAGGACAAAGTGGCTGACTGGTCGCTGGGCGAGGCCTTCGCCTTCGGCTCGCTCCTTAA GGAGGGCATCCACGTTCGACTATCCGGCCAAGATGTGGAGCGCGGCACCTTTTCGCACCGGCACCACGTCCTGCACCACC AGTCGGAGGACAAGGTGGTGTACAACTCACTGGACCACCTCTATCCCGACCAGGCGCCCTACTCCGTCTCCAACAGCTCC CTGTCGGAGTGTGCGGTCCTTGGCTTCGAGCACGGCTACTCCATGGCCAGTCCCAATGCTCTGGTCATGTGGGAAGGCCA GTTTGGGGACTTCTGCAACACGGCGCAGTGCATCATCGACACGTTCATAGCCAGCGGCGAAACCAAGTGGGTGCGCCAGT CCGGAGTCGTCATGTTGCTGCCACACAGCATGGAGGGCATGGGCCCGGAGCACTCTTCCGGCCGCATTGAGCGCTTCCTG CAGATGAGCGACGACGATCCGGACGTCTATCCGGATACCTGCGACGCTGACTTTGTGGCTCGCCAGCTAATGAATGTCAA CTGGATTGTGACGAATCTCTCCACGCCCGCAAATCTGTTCCACTGCCTGCGCCGCCAGGTGAAGATGGGCTTCCGGAAGC CGCTGATTAACTTCTCGCCCAAGTCACTGCTCCGCCATCCGCTGGCGCGAAGTCCCTTCAAGGACTTCAACGAGTGCAGT TGCTTCCAGCGCATCATTCCCGACAAAGGACCCGCCGGCAAGCAGCCCGACTGCGTCCAGAAGCTGGTCTTCTGCTCGGG AAAGGTCTACTACGATCTCGTCAAGGAGCGCGACGATCACGAGCAGGTGGAGACGGTGGCCCTGGTGAGGGTGGAACAGG TGAGACGCCTCCCAACGCGACACCTCTTCTTGTTCATGATAACCATGATTGGGCGCATCCTCCAGCTCTGTCCCTTCCCC TACGACCTGATCAGCCAGCAGCTGGAGCTGTATCCCAAGGCGGAGCTGCTGTGGGCCCAGGAGGAGCACAAGAACATGGG CGCCTGGTCTTACGTGCAGCCGCGTTTCGACACGGCGCTCCTCAAGAACGAAAACGAATCGCGCTGCGTGTCCTATCACG GACGACCGCCCAGCGCCTCGCCAGCGACGGGCAACAAGGTGCAGCACTACAACGAGTACAAGGCCCTGATCACAAGCATC TTCGGAGAGCTGACGCCGGAGAACAAGAAGCGAATCGAGGATCGGATCAAGAAGCAGCAGGCAAAGGCCAAGGCGGATGC GCAATCGAAACCCAGTACTAAGCCACCAGCCGCTCCACCAAAGGGCGGTTCGTCGCCGCCACCAGGTAAGGGCGAATCTG GAGGAAAAAAGAGATCCGATTCGTCTCCATCTGCGTCTATAGCTCCTACAGGTCCAGCACCCCGTAAGCCACTGATCAGT CTGCCAAGTAGACCTCCAAGGGGTGGAAAGCAGCGGTCCGGCTCGGCTCCAGCTCCTAATCTGGTTGATGAAGATTCAGC TGCTCGAAATAGGGAACCAGATGCTTCTGCATATGATGGCGCATCACCGTCAGCTTGGAAGAGATCGGGCTCTGCTCCAG CACCGAGTGTAGTTCCCAGGGCATCGGCCTCCCGCAGTCCGTCTAGAAAGTCACCTTCACCCTCTCACGACGGCATATCT CCTTCTACAAGACAAGAATCCTTAAGCAAAAAGAAATCAGGGTCGGCTCCAGCTCCGACGCCAACTTCTCGTGGACCATT CCGGACTGCGCCCTCCAATACAGAATTCATAAAGCGCAGACCTGCTAAAGATTACGGCAGTAGAAATGAAACCCAAAGCA CTCCCGGGGAGTCGGAACTGGACCCAACAAAACCACAGCCCTAGTTTGAATCCTCCTTTGTAAGTCCACCAATAAAGATA GGTAATAGTTACAGAAAGAAAAAAAAAAAAAAAAAA
Cloned cDNAs prepared from polyadenylated RNA isolated from adult heads.
Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.
RNA from adult heads was poly(A)+ selected once. Oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.
The GH library is separate from the HL library. Since many of the HL clones were not full length and others lacked inserts, a new adult head library was made.
