FB2025_01 , released February 20, 2025
Clone: Dmel\GM03514
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General Information
Symbol
Dmel\GM03514
Species
D. melanogaster
Name
GM03514
FlyBase ID
FBcl0137495
Feature type
cDNA_clone
Computed gene(s)
Dmel\CG5080
 
Collection Status
BDGP
Known Problems
none
FlyBase assessment
    Library
    GM_pBS_cDNA
    • GM cDNA library of D. melanogaster, Oregon-R strain, adult female, ovary, pBS vector.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      oogenesis stage S1 -- S6 & adult stage | female
      ovary
      (Rubin et al., 2000)
      Sequence Data of the Insert
      5' Sequence
      Total bases
      556
      NCBI
      AA801871
      5' sequence dataSequence Downloader
      GACGTCCAGCTCCAAGTTTTCGAGCCAAGTGATCCAAGTGTGTGTGCAAAACAGAGGCTACTCTTCGAACTTGAGCCATT
CAATTTCCCCCAAGGGTCACCCCACGCAGGANCTCGCTTACCAATTTAAGATCGCATCGACGCACCAAAACCCACGATGG
CCGGAGANAGTCGCCCTCTCCAATTAGTTCTCCTGGTCCTAGTTGCGCTCGCCCAATGGCAACCCATCGCCGCTGGATTG
GTCTCCAACGAGGAGCTGCGCGGAACTATCCAGTCGCTGATCTACTCCTACAATCAGCTGGACAATAAGCTGGAGCGGCA
CGAGCATCGCGAGCGTGCGTTGGGCGAGCTGCTCAAGAAGGCGCTGCAGTCGCTCCAGAAGGGCCAGAAGAGCCTGGAGC
CCATCAATGGGATCTTCGGGCGCCTGGACGAGCGGGTCAGCCAGATAGAGACCATGCTGATCAACGCAAGAGGAGAAGTA
CAACTCGCAGTCGGACCGCTTCAACCAGGCAACGGAGCACATGTTCAAGTGGATGCGGGAGAACGACGAGTGCTTC
      Library Information (1)
      Library: GM_pBS_cDNA
      Description

      Cloned cDNAs prepared from polyadenylated RNA isolated from stage S1-S6 ovaries.

      (Rubin et al., 2000)
      Sample preparation

      Ovaries at stage 1-6 of oogenesis were collected from a non-isogenic Oregon R strain P2 population cage.

      Protocol

      RNA from ovaries was poly(A)+ selected once (provided by A. Spradling). cDNA made using Stratagene ZAP-cDNA synthesis kit; oligo(dT) primed with XhoI site at end of primer for first strand synthesis; EcoRI adapter on 5' ends of clones; size fractionated on Sephacryl S-500--approximately 1-6kb. For clones GM01001-GM11496, cDNA directionally cloned into EcoRI/XhoI-digested Stratagene Uni-Zap XR vector, which allows in vivo excision and recircularization of pBluescript SK(+/-) plasmid.

      (Rubin et al., 2000)
      Comment
      Reported As
      Symbol Synonyms
      GM
      (Rubin et al., 2000)
      GM_pBS
      (Rubin et al., 2000)
      GM_pBS_cDNA
      References (1)
      Review (1)
      Rubin et al., 2000, Science 287(5461): 2222--2224
      A Drosophila complementary DNA resource. [FBrf0127297]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)