FB2025_01 , released February 20, 2025
Clone: Dmel\IP17657
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General Information
Symbol
Dmel\IP17657
Species
D. melanogaster
Name
IP17657
FlyBase ID
FBcl0318834
Feature type
cDNA_clone
Computed gene(s)
Dmel\Idh3a
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    IP_cDNA
    • IP cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      839
      NCBI
      EL886405
      3' sequence dataSequence Downloader
      CGAGTGTTTAATTGCTGTTGTTCATACTGCATCATCCTCCTTCTCTGCGCAATATTCCTGGCGTAATTCTCTGCTCTGTT
CTCATCGGTTGTTGTTATGGTTGGCGATACTTGCTACAGCTTATGCGGATGGTCCGTGATCCGTCGTCTACAGCTTGGCA
CAGATCTCGTTCGTGAACTCGGAGCACTTGGCACGGCCGCCCAGATCGCCGGTGAGGTATTTGCCCTCCTTGATGGTCTC
GAAGGCGGCACGCTCGATTTTATCGGCGTACGTGTTGAGCTCCATGTGGCGCAGCATCATGACCGCCGACAGCAACAGGG
CAGTGGGATTGGCCAGGTCCTTGCCGGCAATATCGGGGGCGGTTCCGTGCACAGACTCGAACAGGGCACCGTTCAGGCCC
ATGTTGCCGGAGGGCGTCAGGCCGAGGCCGCCCACCAAACCGGCGCACATGTCAGACAGAATATCACCGTACAGATTGGG
CATGACCAGCACGTCGTATTTTCCGGGGTTCTGGACCATGTTCAGGCACACCGTATCCAGATACTTCTCTTCGAACTGGA
TTTCTGGGAACTTCTGGGCCATGTCGCGGACGCAGCGCAGGAAGAGACCATCGGACATGCGCATAATGTTCGCCTTGTGC
ACCACGGTGACCTTCTTGCGGTTGTTGTTCTTGGCGTACTGGAAGGCGTACTCGGCCACACGCTTGGAGGCTTCCTCGGT
GATCAGCTTGATGCTCTGAACGACGCCGTCGACGATCTCGTGCTCGATGCCGGAGTACTCGCCCTCGGTGTTCTCACGGA
TGGTGACCACGTCCACATCGTCGTACAGCGTCTTGTACC
      5' Sequence
      Total bases
      743
      NCBI
      EL886348
      5' sequence dataSequence Downloader
      CTACCGCTGGCGCAGACTAAAAAGACAGACTTTTTTCTATATAATATACGGCAGGAATCAGTGCAAAAACCGATCCAACG
CAATGGCTGCTAGATTCATACAGAAAATTGTCAACGCGACTCCCGCCGCCAGCCGGTCCTACTCCTCCGGCACCAAGAAA
GTCACCCTCATCCCTGGCGATGGCATCGGACCTGAGATCTCTGCGGCCGTGCAGAAAATCTTCACCGCCGCCAATGTCCC
CATCGAGTGGGAGGCCGTGGACGTGACCCCCGTCCGGGGACCCGACGGAAAGTTCGGCATCCCACTCGATGGGGACATTG
GCGGCGGTGAAGATTTTATGCACGGGAGCGGAGATCTGAGGACCGATGTCATCGAGGGGGATGAGGCTGTGAATCTGGCC
CTGCGCAAGGAGTTCAATCTGTACGCCAACGTGCGTCCCTGCCGCAGTCTCGAGGGCTACAAGACGCTGTACGACGATGT
GGACGTGGTCACCATCCGTGAGAACACCGAGGGCGAGTACTCCGGCATCGAGCACGAGATCGTCGACGGCGTCGTTCAGA
GCATCGAGCTGATCACCGAGGAAGCCTCCAAGCGTGTGGCCGAGTACGCCTTCCAGTACGCCAAGAACAACAACCGCAAG
AAGGTCACCGTGGTGCACAAGGCGAACATTATGCGCATGTCCGATGGTCTCTTCCTGCGCTGCGTCCGCGACATGGCCCA
GAAGTTCCCAGACATCCAGTTCG
      Library Information (1)
      Library: IP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      IP
      IP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)