FB2025_01 , released February 20, 2025
Clone: Dmel\IP15646
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General Information
Symbol
Dmel\IP15646
Species
D. melanogaster
Name
IP15646
FlyBase ID
FBcl0353545
Feature type
cDNA_clone
Computed gene(s)
Dmel\Arr2
 
Collection Status
N/A
Known Problems
none
FlyBase assessment
    Library
    IP_cDNA
    • IP cDNA library of D. melanogaster, isolated by an inverse-PCR screen (SLIP) from other cDNA libraries.
    Strain
      Vector
      Tissue Source
      Stage
      Tissue/Position (including subcellular localization)
      Reference
      embryonic stage
      organism

      Comment: 0-22 hr AEL

      (Rubin et al., 2000)
      larval stage
      organism
      (Rubin et al., 2000)
      pupal stage
      organism

      Comment: early pupae

      (Rubin et al., 2000)
      adult stage
      adult head
      (Rubin et al., 2000)
      Sequence Data of the Insert
      3' Sequence
      Total bases
      761
      NCBI
      EL884329
      3' sequence dataSequence Downloader
      GATAGCCATAACACATTTATAATATAGCGACACGAGGTCTGTTCAACTCATCAACATGCATATGTACGGGTACAAACTCC
AGATCCACCAAAGTTACATTATAGATACGATTATAATACAAAGATTGTGGCCTCCACGAGAGACTGCTGCAACAAATTTT
CAGTGCTTTCGATTTAGTTCAATTCGGTTCACAACAGAGCTAGAAGAGATTGAAGATCGGTGGCCTAGTCCGCCATGTTC
ACATTGTTCATGCGCATCTTGGCGAAGTCCTCGAATACAATATTATCGTCATCATCCTGGTAATAGCCCTTCACATTGCG
GTGCTGCTCGATGGACTTCATTTTCTTCATGGCATTCGAGCGCTTCTTCTCAATGGTTCCAGGTGCCGGTTGGAGTAGCT
TGAAGGGCACATCCGTCTGCATTTCACCTCCCAGAGTGCCGCAGTTGAGCTTAATGCGCACCGAGTACGAGATGACAATA
CCACAGGCATCTCCCGTGGACTTGCCCTCCTGCACCATGGTAGATGAAGCCAGGTTGACATCCTCGTCCTTCAAGTGACC
ATCCAGGGCGATACCATGGCGATCCTTGTTGTTAGCCGCCAGCGGAATCAAGTAGAAGGTCTTGGTGAGATTGGCGCCCG
GAGTGATGGGGCAGCCCTCCTTGGTCTCCAGCTGGGCCACATGCTTGCTGAACTGGGCGTTCACCATGGTGATCTCAGTG
TGCTGCACAATGAAGCACTTGATGCTCTTCACGGACTTCTT
      5' Sequence
      Total bases
      915
      NCBI
      EL884330
      5' sequence dataSequence Downloader
      AAAACTCTATAACGCGCATCGCCCGACTTAGCCTCGAATTGCCCAGTTGCATTTGCCAGGAATCAAAGAAATCCACTAAT
CCACAATGGTTGTATCCGTGAAGGTCTTTAAGAAGGCCACGCCCAACGGCAAGGTCACCTTTTATCTGGGACGCCGCGAC
TTCATCGACCACATCGACTACTGTGATCCCGTCGATGGAGTGATTGTGGTGGAACCGGACTATCTAAAGAACAGGAAAGT
CTTTGGCCAACTGGCCACCACCTATCGCTATGGTCGCGAGGAGGACGAGGTCATGGGAGTGAAGTTCTCCAAGGAGCTTA
TCCTGTGCCGGGAGCAAATTGTGCCCATGACCAATCCCAACATGGAGATGACACCGATGCAGGAGAAGCTGGTCCGCAAA
CTGGGCAGCAACGCCTATCCGTTCACCTTCCACTTCCCGCCCAATTCGCCCAGCTCCGTTACTCTGCAGCAGGAGGGCGA
CGACAATGGCAAGCCCCTGGGCGTCGAGTACACCATCCGGGCATTTGTGGGCGACTCCGAGGACGATCGCCAGCACAAGC
GGAGCATGGTCAGTCTGGTGATCAAGAAGCTGCAGTACGCTCCCCTCAATCGTGGCCAGCGTCTGCCCAGCTCGCTGGTC
AGCAAGGGATTCACCTTCTCGAATGGCAAGATCAGTCTGGAGGTTACGCTGGACAGGGAGATCTACTATCATGGCGAGAA
GACGGCTGCCACCGTCCAGGTGTCGAACAACTCCAAGAAGTCCGTGAAGAGCATCAAGTGCTTCATTGTGCAGCACACTG
AGATCACCATGGTGAACGCCCAGTTCAGCAAGCATGTGGCCCAGCTGGAGACCAAGGAGGGCTGCCCCATCACTCCGGGC
GCCAATCTCACCAAGACCTTCTACTTGATTCCGCT
      Library Information (1)
      Library: IP_cDNA
      Description

      Clones isolated by an inverse-PCR screen (SLIP) using a mixture of the GH, LD, LP, and SD cDNA libraries.

      Sample preparation

      Adult heads were collected from an isogenic y; cn bw sp (iso-1) strain.

      Protocol

      These clones have been isolated a directed screening process called SLIP (Self-Ligation of Inverse PCR Products). Clones isolated from this screen have been PCR-amplified using a mixture of 4 different plasmid cDNA libraries as template. These include the GH, LD, LP, and SD cDNA libraries.

      (FlyBase Curators, 2016-)
      Comment
      Reported As
      Symbol Synonyms
      IP
      IP_cDNA
      References (1)
      FlyBase analysis (1)
      FlyBase Curators, 2016-, Curation of large dataset metadata by FlyBase.
      Curation of large dataset metadata by FlyBase. [FBrf0232540]
      External Crossreferences and Linkouts ( 1 )
      Linkouts
      DGRC - Stock center for Drosophila cDNAs, vectors, and cell lines
      Synonyms and Secondary IDs (0)
      Reported As
      Symbol Synonym
      Secondary FlyBase IDs
        References (0)