FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hus1-like, Rad1
FlyBase ID
FBig0000065264
Interaction Network
Interactions Browser links
Hus1-like network
Rad1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0193459-1.Y2H
Reference
Abdu et al., 2007
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hus1-like 

Hus1

bait

fused to LexA DNA-binding domain

Rad1 

Rad1

prey

fused to B42 activation domain

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Abdu et al., 2007)

Two-hybrid system: yeast LexA-BD/B42-AD

(Abdu et al., 2007)
FBrf0218395-4709.DPiM
Reference
Guruharsha et al., 2011
Description
physical association
Assay
Collection
DPiM-2
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hus1-like 
unspecified role
Hus1-like 
unspecified role
Hus1-like 
unspecified role
Rad1 
unspecified role
Rad1 
unspecified role
Rad1 
unspecified role
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

HGScore = 86.46259

FBrf0218513-1.coIP.WB
Reference
Kadir et al., 2012
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
Rad1 

DmRad1

bait

GFP tag

Hus1-like 

Hus1

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Bait expressed in transfected cells.

(Kadir et al., 2012)

Prey expressed in transfected cells.

(Kadir et al., 2012)

Rad1, Rad9 and Hus1-like interact as part of the 9-1-1 complex.

(Kadir et al., 2012)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)