FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
γTub37C, Grip163
FlyBase ID
FBig0000097993
Interaction Network
Interactions Browser links
γTub37C network
Grip163 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0107918-6.coIP.MW
Reference
Oegema et al., 1999
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage 1embryonic stage 8blastoderm stageembryonic stage 5embryonic stage 3embryonic cycle 6embryonic cycle 8embryonic cycle 5embryonic stage 6embryonic cycle 7embryonic cycle 4embryonic cycle 3embryonic stage 4embryonic stage 7embryonic stage 2embryonic cycle 9
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γtub

bait
Grip163 

Grip163

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Oegema et al., 1999)

γTub37C and Grip163 coimmunoprecipitate and cosediment as part of the γTuRC (γ-tubulin ring complex).

(Oegema et al., 1999)
FBrf0159185-16.coIP.WB
Reference
Gunawardane et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γ-tubulin

bait
Grip163 

Grip163

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gunawardane et al., 2003)
FBrf0159185-23.CS.WB
Reference
Gunawardane et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Grip163 

Grip163

neutral component
Grip163 

Grip163

neutral component
Grip163 

Grip163

neutral component
γTub37C 

γ-tubulin

neutral component
γTub37C 

γ-tubulin

neutral component
γTub37C 

γ-tubulin

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts of wild-type fly line; proteins produced from endogenous gene.

(Gunawardane et al., 2003)
FBrf0159185-52.coIP.MW
Reference
Gunawardane et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γtubulin

bait
Grip163 

Grip163

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gunawardane et al., 2003)
FBrf0159185-53.coIP.MW
Reference
Gunawardane et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Grip163 

Grip163

bait
γTub37C 

γtubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gunawardane et al., 2003)
FBrf0159185-56.coIP.WB
Reference
Gunawardane et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γtubulin

bait
Grip163 

Grip163

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gunawardane et al., 2003)
FBrf0159185-57.coIP.MW
Reference
Gunawardane et al., 2003
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Grip163 

Grip163

bait
γTub37C 

γtubulin

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of Sf9 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Gunawardane et al., 2003)
FBrf0159185-6.coIP.MW
Reference
Gunawardane et al., 2003
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γ-tubulin

bait
Grip163 

Grip163

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryonic extracts of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Gunawardane et al., 2003)
FBrf0201798-30.CoIP.WB
Reference
Wiese, 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γTub37CD

bait
Grip163 

Dgrip163

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)
FBrf0201798-34.CS.WB
Reference
Wiese, 2008
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γTub37CD

bait
Grip163 

Dgrip163

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Wiese, 2008)
FBrf0219801-34.CS.WB
Reference
Gopalakrishnan et al., 2012
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γ-tubulin

neutral component
γTub37C 

γ-tubulin

neutral component
γTub37C 

γ-tubulin

neutral component
Grip163 

Grip163

neutral component
Grip163 

Grip163

neutral component
Grip163 

Grip163

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; proteins produced from endogenous gene.

(Gopalakrishnan et al., 2012)

γTub37C and Grip163 co-fractionate as part of a Sas-4-containing S-γ-TuRC (γ-tubulin ring) complex.

(Gopalakrishnan et al., 2012)

Sas-4 complexes were immunoprecipitated from "high-speed lysates" depleted of mature centrosomes and different Sas-4-containing complexes were fractionated on a sucrose gradient.

(Gopalakrishnan et al., 2012)
FBrf0244583-9.coIP.MW
Reference
Tariq et al., 2020
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
γTub37C 

γ-Tub

bait

GFP tag

Grip163 

Grip163

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Tariq et al., 2020)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (5)