FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
BicD, Chc
FlyBase ID
FBig0000098970
Interaction Network
Interactions Browser links
BicD network
Chc network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0210180-1.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
embryonic/larval hypodermal muscle cell / third instar larval stagelarval peripheral nervous system / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

BicD

bait
Chc 

Chc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval neuromusculature of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2010)
FBrf0210180-2.Y2H
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chc 

Chc

bait

fused to GAL4 DNA-binding domain

Chc 
BicD 

BicD

prey

fused to GAL4 activation domain

BicD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BicD 
C-terminal domain
sufficient binding region
aa 536-782
Chc 
Ankle domain
sufficient binding region
aa 330-807
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Li et al., 2010)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Li et al., 2010)
FBrf0210180-3.coIP.MS
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

BicD

bait
Chc 

Chc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2010)

Immunoprecipitation of BicD led to specific capture of BicD and only one other protein visible by Coomassie staining, which had a molecular weight of 190 kDa and was determined to be Chc by mass spectrometry.

(Li et al., 2010)
FBrf0210180-4.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

BicD

bait
Chc 

Chc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Li et al., 2010)
FBrf0210180-5.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

BicD

bait

Protein A tag

Chc 

Chc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2010)
FBrf0210180-6.coIP.WB
Reference
Li et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult head / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chc 

Chc

bait

HA tag

BicD 

BicD

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult heads of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Li et al., 2010)
FBrf0224776-1.coIP.MS
Reference
Vazquez-Pianzola et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

Bic-D

bait
Chc 

Chc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2014)
FBrf0224776-3.coIP.WB
Reference
Vazquez-Pianzola et al., 2014
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

Bic-D

bait
Chc 

Chc

prey

Myc tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Vazquez-Pianzola et al., 2014)
FBrf0224776-5.coIP.WB
Reference
Vazquez-Pianzola et al., 2014
Description
physical association
Assay
Collection
Source/Stage
ovary / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

Bic-D

bait
Chc 

Chc

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult female ovaries of wild-type fly line; bait produced from endogenous gene; prey produced from endogenous gene.

(Vazquez-Pianzola et al., 2014)
FBrf0253939-2.Y2H
Reference
Vazquez-Pianzola et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Chc 

Chc

bait

fused to GAL4 DNA-binding domain

Chc 
BicD 

BicD

prey

fused to GAL4 activation domain

BicD 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
BicD 
CTD domain
sufficient binding region
unspecified
Chc 
interaction domain
sufficient binding region
aa 329-803
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Vazquez-Pianzola et al., 2022)

Two-hybrid system: yeast GAL4-BD/GAL4-AD; addition of tacc increased interaction between BicD and Chc.

(Vazquez-Pianzola et al., 2022)
FBrf0253939-6.coIP.WB
Reference
Vazquez-Pianzola et al., 2022
Description
physical association
Assay
Collection
Source/Stage
organism / embryonic stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicD 

BicD

bait
Chc 

Chc

prey

FLAG tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was embryos of transgenic fly line; bait produced from endogenous gene; prey produced from tagged transgenic construct.

(Vazquez-Pianzola et al., 2022)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)