Interaction in vitro; bait peptide synthesized in vitro; prey derived from wild-type embryonic extract.

Sfmbt binds to His3 histone tail that is monomethylated at Lys 9 (Kd = 7 uM) or dimethylated at Lys 9 (Kd = 9 uM). His3 peptides that are unmodified or trimethylated at Lys 9 bind with over ten-fold reduced affinity. No binding was observed for His3 peptides methylated at residues Lys 4 or Lys 27.

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey peptide synthesized in vitro.

Sfmbt binds to various mono- and dimethylated lysines in His3 and His4 with very low micromolar affinity, but not to unmethylated or trimethylated peptides.

The lack of Sfmbt specificity for different His3 and His4 mono- or dimethylated lysine residues observed in this study contrasts with work in Klymenko et al., 2006 (FBrf0190547) and may reflect technical differences.

Interaction in vitro; bait peptide synthesized in vitro; prey produced as a recombinant fusion protein in bacterial system.

Sfmbt binds to various mono- and dimethylated lysines in His3 and His4 with very low micromolar affinity, but not to unmethylated or trimethylated peptides.

The lack of Sfmbt specificity for different His3 and His4 mono- or dimethylated lysine residues observed in this study contrasts with work in Klymenko et al., 2006 (FBrf0190547) and may reflect technical differences.