FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
p38b, Rack1
FlyBase ID
FBig0000100895
Interaction Network
Interactions Browser links
p38b network
Rack1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0223779-1.coIP.MS
Reference
Belozerov et al., 2014
Description
physical association
Assay
Collection
Source/Stage
thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p38b 

p38b

bait

FLAG tag

p38b 
Rack1 

Rack1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
p38b 
kinase domain
mutation increasing interaction
aa 53

K53R, \'KD\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Belozerov et al., 2014)

A kinase dead version of p38b was used to trap substrates.

(Belozerov et al., 2014)
FBrf0223779-2.coIP.WB
Reference
Belozerov et al., 2014
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
p38b 

p38b

bait

FLAG tag

p38b 
Rack1 

Rack1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
p38b 
kinase domain
mutation increasing interaction
aa 53

K53R, \'KD\'

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Belozerov et al., 2014)

The interaction of Rack1 with p38b could only be detected when a kinase dead version of p38b was used as bait.

(Belozerov et al., 2014)
FBrf0223779-3.coIP.WB
Reference
Belozerov et al., 2014
Description
physical association
Assay
Collection
Source/Stage
thorax / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
p38b 

p38b

bait

FLAG tag

Rack1 

Rack1

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adults of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Belozerov et al., 2014)

The interaction of Rack1 with p38b is enhanced in aging flies (30 days versus 5 days of age).

(Belozerov et al., 2014)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (1)