Interaction in vitro; fluorescence donor produced as a recombinant fusion protein in bacterial system and labeled in vitro; fluorescence acceptor produced as a recombinant fusion protein in bacterial system and labeled in vitro.
Dissociation constant of Kd=1942nM.
Interactions between bZIP proteins were quantified in vitro using a solution FRET assay at 4oC, 21oC and 37oC. Only interactions at 21oC with Kd < 5000 nm were curated by FlyBase.
