Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

Evi5 specifically binds the GTP-bound state of Rab6 and Rab14 (not the GDP-locked forms).

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

Evi5 interacts with the GTP-locked form of Rab6, and more weakly with the GTP-locked form of Rab14 (not the GDP-locked forms).

Two-hybrid system: yeast GAL4-BD/GAL4-AD

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from S2 cell detergent-free extract (endogenous gene).

Specificity S-score=15.17.

Constitutively active (GTP-locked) form of Rab was used for pull down. Parallel pull downs performed for several Rabs proteins in this study. Yield was measured using spectral counts. A specificity score (S score) was calculated, where greater weight was given to interactions seen with fewer baits.