FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Cpsf100, Sym
FlyBase ID
FBig0000106035
Interaction Network
Interactions Browser links
Cpsf100 network
Sym network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0208078-4.coIP.WB
Reference
Sullivan et al., 2009
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-ThermoFischer
Participants
Corresponds to
Reported as
Role
Note
Sym 

Symplekin

bait
Cpsf100 

CPSF100

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from endogenous gene; prey produced from endogenous gene.

(Sullivan et al., 2009)
FBrf0229062-2.coIP.WB
Reference
Michalski and Steiniger, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sym 

Symp

bait

HA tag

Sym 
Cpsf100 

CPSF100

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sym 
interaction domain
sufficient binding region
aa 272-1080

coordinates relative to Sym-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Sym, Cpsf73 and Cpsf100 were co-transfected.

(Michalski and Steiniger, 2015)

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Michalski and Steiniger, 2015)
FBrf0229062-3.coIP.WB
Reference
Michalski and Steiniger, 2015
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Cpsf100 

CPSF100

bait

HA tag

Cpsf100 
Sym 

Symp

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Cpsf100 
C-terminal region
sufficient binding region
aa 525-756

coordinates relative to Cpsf100-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Michalski and Steiniger, 2015)

Sym, Cpsf73 and Cpsf100 were co-transfected.

(Michalski and Steiniger, 2015)
FBrf0235253-11.coIP.WB
Reference
Harrington et al., 2017
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Sym 

Sym

bait

HA tag

Sym 
Cpsf100 

Cpsf100

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Sym 
interaction domain
sufficient binding region
aa 272-1165
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Harrington et al., 2017)
External Crossreferences and Linkouts ( 1 )
Linkouts
MIST - An integrated Molecular Interaction Database
References (3)