FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
GNBP3, PPO1
FlyBase ID
FBig0000115170
Interaction Network
Interactions Browser links
GNBP3 network
PPO1 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0210659-1.coIP.WB
Reference
Matskevich et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult hemolymph / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
GNBP3 

GNBP3

bait
PPO1 

PPO

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult hemolymph of wild-type, mutant or transgenic fly line; bait produced from endogenous gene or transgenic construct; prey produced from endogenous gene.

(Matskevich et al., 2010)

GNBP3 associates with uncleaved PPO1 in unchallenged flies and with the cleaved and active form of PPO1 in flies injected with B. bassiana. A GNBP3-PPO1 association is not observed in flies naturally infected with B. bassiana.

(Matskevich et al., 2010)
FBrf0210659-2.CL
Reference
Matskevich et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult hemolymph / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
GNBP3 

GNBP3

neutral component
GNBP3 

GNBP3

neutral component
GNBP3 

GNBP3

neutral component
PPO1 

PPO

neutral component
PPO1 

PPO

neutral component
PPO1 

PPO

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was adult hemolymph of wild-type fly line; proteins produced from endogenous gene.

(Matskevich et al., 2010)

Antibodies to both GNBP3 and PPO1 recognize a high molecular weight (180-190kDa) moiety in hemolymph after crosslinking.

(Matskevich et al., 2010)
FBrf0210659-3.AF.WB
Reference
Matskevich et al., 2010
Description
physical association
Assay
Collection
Source/Stage
adult hemolymph / adult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
GNBP3 

GNBP3

bait
PPO1 

PPO

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey derived from mutant adult hemolymph.

(Matskevich et al., 2010)
External Crossreferences and Linkouts ( 0 )
References (1)