FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Hipk, hbn
FlyBase ID
FBig0000119769
Interaction Network
Interactions Browser links
Hipk network
hbn network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0242157-1.BiFC.FM
Reference
Steinmetz et al., 2019
Description
physical association
Assay
Collection
Source/Stage
wing disc / third instar larval stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hipk 

Hipk

bait

YFP tag, N-terminal fragment

hbn 

Hbn

prey

YFP tag, C-terminal fragment

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was larval wing discs of transgenic fly line; proteins produced from transgenic constructs.

(Steinmetz et al., 2019)
FBrf0242157-4.ENZ.A
Reference
Description
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Hipk 

Hipk

enzyme

GST tag

Hipk 

Hipk

enzyme

GST tag

hbn 

Hbn

enzyme target

GST tag, P[32] label

hbn 

Hbn

enzyme target

GST tag, P[32] label

hbn 
hbn 
hbn 
hbn 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction
External Crossreferences and Linkouts ( 0 )
References (1)