FB2025_01 , released February 20, 2025
Physical Interaction report
Open Close
General Information
Interaction Type
physical association
Interacting Genes
mir-8, VhaAC45
FlyBase ID
FBig0000120382
Interaction Network
Interactions Browser links
mir-8 network
VhaAC45 network
esyN Network Diagram
Show neighbor-neighbor interactions:
Select Layout:
Legend:
Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0245520-12.miR-mRNA.LUC
Reference
Lim et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
mir-8 
VhaAC45 

VhaAC45

prey
VhaAC45 
VhaAC45 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
VhaAC45 
3-prime UTR
sufficient binding region
VhaAC45 
mir-8 target site
mutation decreasing interaction
CACCGACGCAGCAUCCAGUAUUU to CACCGACGCAGCAUCCAUAUGUU
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Luciferase reporter assay.

(Lim et al., 2020)

Source was S2 cell line; bait produced from transfected construct; prey produced from transfected reporter construct.

(Lim et al., 2020)
FBrf0245520-17.miR-mRNA.PCR
Reference
Lim et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
mir-8 

miR-8

bait
VhaAC45 

VhaAC45

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Overexpression of mir-8 reduces VhaAC45 mRNA levels.

(Lim et al., 2020)

Source was S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lim et al., 2020)
External Crossreferences and Linkouts ( 0 )
References (1)