FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
IntS11, IntS9
FlyBase ID
FBig0000120752
Interaction Network
Interactions Browser links
IntS11 network
IntS9 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0247102-27.coIP.MS
Reference
Huang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

bait

FLAG tag

IntS9 

IntS9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2020)
FBrf0247102-57.coIP.WB
Reference
Huang et al., 2020
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-DGRC
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

bait

FLAG tag

IntS9 

IntS9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was nuclear extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Huang et al., 2020)
FBrf0254630-5.PD.WB
Reference
Lin et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

neutral component

FLAG tag

IntS11 

IntS11

neutral component

FLAG tag

IntS11 

IntS11

neutral component

FLAG tag

IntS9 

IntS9

neutral component
IntS9 

IntS9

neutral component
IntS9 

IntS9

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of DL1 cell line; bait produced from transfected construct. Prey produced from endogenous gene.

(Lin et al., 2022)
FBrf0254630-9.PD.WB
Reference
Lin et al., 2022
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS9 

IntS9

neutral component

FLAG tag

IntS9 

IntS9

neutral component

FLAG tag

IntS9 
IntS9 

IntS9

neutral component

FLAG tag

IntS9 
IntS11 

Int11

neutral component
IntS11 

Int11

neutral component
IntS11 

Int11

neutral component
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
IntS9 
inositol hexakisphosphate binding residue 2
mutation decreasing interaction
aa 2

R2E; coordinates relative to IntS9-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of DL1 cell line; bait produced from transfected construct. Prey produced from endogenous gene.

(Lin et al., 2022)
FBrf0260214-33.coIP.WB
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

bait

FLAG tag

IntS9 

IntS9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lin et al., 2024)
FBrf0260214-38.coIP.MS
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

bait

FLAG tag

IntS9 

IntS9

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Lin et al., 2024)
FBrf0260214-46.PD.CH.MW
Reference
Lin et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
IntS11 

IntS11

neutral component

His tag

IntS11 

IntS11

neutral component

His tag

IntS11 

IntS11

neutral component

His tag

IntS9 

IntS9

neutral component

His tag

IntS9 

IntS9

neutral component

His tag

IntS9 

IntS9

neutral component

His tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; proteins produced as recombinant fusion proteins in baculovirus and insect cell system.

(Lin et al., 2024)

IntS11, IntS9 and Brat1 form a heterotrimer in vitro. Captured as three pairwise interactions.

(Lin et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (3)