FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
gnu, BicC
FlyBase ID
FBig0000121214
Interaction Network
Interactions Browser links
gnu network
BicC network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0249567-1.coIP.MS
Reference
Avilés-Pagán et al., 2021
Description
physical association
Assay
Collection
Source/Stage
stage S14 oocyte / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gnu 

GNU

bait

GFP tag

gnu 
gnu 
BicC 

BIC-C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gnu 
SAM domain
necessary binding region
aa 172-240

coordinates relative to gnu-PA

gnu 
CDK1 phosphorylation sites
mutation decreasing interaction
aa 13,16,19,32,92,156,158,170,178

S13A,T16A,T19A,T32A,T92A,S156A,T158A,S170A,S178A, coordinates relative to gnu-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was oocytes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Avilés-Pagán et al., 2021)
FBrf0249567-3.coIP.WB
Reference
Avilés-Pagán et al., 2021
Description
physical association
Assay
Collection
Source/Stage
stage S14 oocyte / femaleadult stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
gnu 

GNU

bait

GFP tag

gnu 
gnu 
BicC 

BIC-C

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gnu 
SAM domain
necessary binding region
aa 172-240

coordinates relative to gnu-PA

gnu 
CDK1 phosphorylation sites
mutation decreasing interaction
aa 13,16,19,32,92,156,158,170,178

S13A,T16A,T19A,T32A,T92A,S156A,T158A,S170A,S178A, coordinates relative to gnu-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

The gnu-BicC interaction is maintained after in vitro egg activation.

(Avilés-Pagán et al., 2021)

Source was oocytes of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.

(Avilés-Pagán et al., 2021)
FBrf0249567-5.PD.WB
Reference
Avilés-Pagán et al., 2021
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
BicC 

BIC-C

bait

GST tag

gnu 

GNU

prey

MBP tag

gnu 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
gnu 
SAM domain
necessary binding region
aa 172-240

coordinates relative to gnu-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced as a recombinant fusion protein in bacterial system.

(Avilés-Pagán et al., 2021)
External Crossreferences and Linkouts ( 0 )
References (1)