Mutation of nt 6-11 to purines within the context of nt 6-48.
Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; wild type prey bait from synthesized oligonucleotides; purine mutated prey produced by in vitro transcription; wild type and mutant prey radioactively capped.
Interaction increased with RNAi knockdown of ais.
Source was embryos of transgenic fly line; bait produced from tagged transgenic construct; prey produced from endogenous gene.
