Interaction in vitro; protein produced as a recombinant fusion protein in bacterial system; RNA in vitro transcribed and labeled.
The snoRNA was synthesised as two differently labelled RNA fragments (G980 part1 and G980 part2) that retained the overall secondary structures. Df31 binds with higher affinity to the part2 of snoRNA:Me28S-G980 with a Kd of 7um.
Source was embryos of wild type fly line; bait and prey produced from endogenous gene.
