FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
Tet, Bap55
FlyBase ID
FBig0000123162
Interaction Network
Interactions Browser links
Tet network
Bap55 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0259266-1.coIP.MS
Reference
Tu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Tet 

Tet

bait

FLAG tag

Bap55 

Bap55

prey
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from endogenous gene.

(Tu et al., 2024)
FBrf0259266-2.coIP.WB
Reference
Tu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
S2-unspecified
Participants
Corresponds to
Reported as
Role
Note
Tet 

Tet

bait

FLAG tag

Tet 
Tet 
Bap55 

Bap55

prey

HA tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tet 
C-terminal F1 domain
sufficient binding region
unspecified

coordinates relative to Tet-PF

Tet 
C-terminal F4 domain
sufficient binding region
unspecified

coordinates relative to Tet-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Source was cell extract of S2 cell line; bait produced from transfected construct; prey produced from transfected construct.

(Tu et al., 2024)
FBrf0259266-5.coIP.WB
Reference
Tu et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Tet 

Tet

bait

Myc tag

Tet 
Bap55 

Bap55

prey

Strep tag

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Tet 
C-terminal domain
sufficient binding region
aa 1785-2921

coordinates relative to Tet-PF

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion proteins in baculovirus and insect cell system; prey produced as a recombinant fusion protein in bacterial system.

(Tu et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)