FB2025_01 , released February 20, 2025
Physical Interaction report
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General Information
Interaction Type
physical association
Interacting Genes
t-Grip84, Grip91
FlyBase ID
FBig0000123331
Interaction Network
Interactions Browser links
t-Grip84 network
Grip91 network
esyN Network Diagram
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Protein
RNA
Selected Interactor(s)
Common Interactor(s)
Reported Interactions
FBrf0260790-3.Y2H
Reference
Alzyoud et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
Grip91 

Grip91

bait

fused to GAL4 DNA-binding domain

Grip91 
t-Grip84 

t-Grip84

prey

fused to GAL4 activation domain

t-Grip84 
Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Grip91 
N-terminal domain
sufficient binding region
aa 1-545

coordinates relative to Grip91-PA

t-Grip84 
C-terminal domain
sufficient binding region
aa 400-728

coordinates relative to t-Grip84-PA

Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Two-hybrid system: yeast GAL4-BD/GAL4-AD

(Alzyoud et al., 2024)

Source was yeast cell line; bait produced as transgenic fusion protein; prey produced as transgenic fusion protein (prey was previously cloned reagent).

(Alzyoud et al., 2024)
FBrf0260790-9.PD.A
Reference
Alzyoud et al., 2024
Description
physical association
Assay
Collection
Source/Stage
Cell line used
Participants
Corresponds to
Reported as
Role
Note
t-Grip84 

t-Grip84

bait

GST tag

Grip91 

Grip91

prey

[35]S label

Experimental entities
Corresponds to
Identifier
Reported
Role
Note
Subregions with role in interaction
Corresponds to
Description
Role
Coordinates
Note
Isoform-specific participants
Corresponds to
Description
Role
Note
Comments concerning this interaction

Interaction in vitro; bait produced as a recombinant fusion protein in bacterial system; prey produced and labeled by in vitro translation.

(Alzyoud et al., 2024)
External Crossreferences and Linkouts ( 0 )
References (1)